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US 6,048,732 AGranted
Receptor and method for citrate determination
AssigneeBoard of Regents, The University of Texas System
InventorsEric V. Anslyn, Axel Metzger
Life of this patent
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Abstract
A sensing composition for polycarboxylic acids which operates in competitive solvents. The composition includes a receptor and an indicator, both of which are mixed in a solvent. Addition of a polycarboxylic acid to the composition causes a change in the physical properties of the composition. Measurement of the amount of change in solution allows the concentration of polycarboxylic acid to be determined. The sensor is preferably selective for polycarboxylic acids, is sensitive, and may be used to analyze solutions of polycarboxylic acids.
Claims22
A method for determining a concentration of a polycarboxylic acid in a solution, the method comprising:
- adding the solution comprising the polycarboxylic acid to a composition, the composition comprising a receptor for the polycarboxylic acid, an indicator, and a solvent, the receptor being adapted to bind to the polycarboxylic acid during use, the indicator being adapted to bind to the receptor such that at least one physical property of the indicator are altered during use, wherein the receptor comprises a compound of the formula:
- (R.sup.1).sub.n --X--(R.sup.2).sub.m
- wherein X comprises carbocyclic systems or C1-C10 alkanes, n is an integer of at least 2, m is an integer of at least 1;
- and
- wherein each of R.sup.1 independently represents --(CH2).sub.y --NR.sup.3 --C(NR.sup.4)--NR.sup.5, or --(CH.sub.2).sub.y --NR.sup.6 R.sup.7 ;
- where y is an integer of at least 1;
- where R.sup.3, R.sup.4, and R.sup.5 independently represent hydrogen, alkyl, aryl, alkyl carbonyl of 1 to 10 carbon atoms, or alkoxy carbonyl of 1 to 10 carbon atoms, or R.sup.4 and R.sup.5 together represent a cycloalkyl group,
- where R.sup.6 represents hydrogen, alkyl, aryl, alkyl carbonyl of 1 to 10 carbon atoms, or alkoxy carbonyl of 1 to 10 carbon atoms;
- where R.sup.7 represents hydrogen, alkyl, aryl, alkyl carbonyl of 1 o 10 carbon atoms, or alkoxy carbonyl of 1 to 10 carbon atoms;
- or where R.sup.6 and R.sup.7 together represent a cycloalkyl group;
- and
- wherein each of R.sup.2 independently represents hydrogen, alkyl, alkenyl, alkynyl, phelnyl, phenylalkyl, arylalkyl aryl, or together with another group represent a carbocyclic ring;
- and
- wherein n is at least equal to the number of carboxylic acid groups of the polycarboxylic acid;
- allowing the polycarboxylic acid to interact with the receptor to substantially release the indicator from the receptors;
- moitoring the change in the physical property of the indicator after the addition of the solution;
- and calculating the concentration of the polycarboxylic acid in the solution based on the change in physical property of the indicator.
14 more elements
A method for determining a concentration of citric acid in a solution, the method comprising:
- adding the solution comprising the citric acid to a composition, the composition comprising a receptor for the citric acid, an indicator, and a solvent, the receptor being adapted to bind to the citric acid during use, the indicator beings adapted to bind to the receptor such that at least one physical property of the indicator are altered during use, wherein the receptor comprises a compound of the formula:
- (R.sup.1).sub.n --X--(R.sup.2).sub.m
- wherein X comprises carbocyclic systems or C.sub.1 -C.sub.10 alkanes, n is an integer of at least 3, m is an integer of at least 1;
- and
- wherein each of R.sup.1 independently represents --(CH2).sub.y --NR.sup.3 --C(NR.sup.4)--NR.sup.5, or --(CH.sub.2).sub.y --NR.sup.6 R.sup.7 ;
- where y is an integer of at least 1;
- where R.sup.3, R.sup.4, and R.sup.5 independently represent hydrogen, alkyl, aryl, alkyl carbonyl of 1 to 10 carbon atoms, or alkoxy carbonyl of 1 to 10 carbon atoms, or R.sup.4 and R.sup.5 together represent a cycloalkyl group;
- where R.sup.6 represents hydrogen, alkyl, aryl, alkyl carbonyl of 1 to 10 carbon atoms, or alkoxy carbonyl of 1 to 10 carbon atoms;
- where R.sup.7 represents hydrogen, alkyl, aryl, alkyl carbonyl of 1 to 10 carbon atoms, or alkoxy carbonyl of 1 to 10 carbon atoms;
- or where R.sup.6 and R.sup.7 together represent a cycloalkyl group;
- and
- wherein each of R.sup.2 independently represents hydrogen, alkyl, alkenyl, alkynyl, phenyl, phenylalkyl, arylalkyl aryl, or together with another group represent a carbocyclic ring;
- allowing the polycarboxylic acid to interact with the receptor to substantially release the indicator from the receptor;
- moitoring the change in the physical property of the indicator after the addition of the solution;
- and
- calculating the concentration of the citric acid in the solution based on the change in physical property of the indicator.
13 more elements
Dependencies are inferred from the claim text.