Bioreactor for mammalian cell growth and maintenance

A a bioreactor system for ex vivo maintenance and growth of mammalian cells, comprising:

• a central processing unit;
• a transportable cassette, separable from said central processing unit having,
• a data path to said central processing unit;
• a bioreactor having a cell growth chamber and a gas chamber;
• a computer storage medium encoded with a computer code mechanism that when executed by the central processing unit through said data path controls environmental conditions in the bioreactor to be suitable for the ex vivo maintenance and growth of the mammalian cells, said computer storage medium being encoded with,
• a media flow rate parameter embedded therein and being accessible via said data path to said central processing unit when maintaining a predetermined media flow rate through the cell growth chamber so as to maintain the environmental conditions to be suitable for the maintenance and growth of the mammalian cells;
• and
• means for monitoring process data while said mammalian cells are maintained and grown in said bioreactor.

A computer-controlled bioreactor for ex vivo maintenance and growth of mammalian cells, comprising:

• a transportable cassette having
• a cell growth chamber having a media inlet, a media outlet and an internal volume;
• a gas permeable, liquid impervious member disposed within said internal volume so as to divide said internal volume into a gas chamber and a cell growth chamber;
• a computer storage medium encoded with a computer code mechanism that when executed by a processor that is external to said transportable cassette controls environmental conditions suitable for the ex vivo maintenance and growth of the mammalian cells, said computer program code mechanism including,
• a media flow code mechanism having a media flow rate parameter embedded therein and being accessible to said processor when maintaining a predetermined media flow rate through the cell growth chamber by way of said media inlet.