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US 6,048,691 AGranted
Method and system for performing a binding assay
AssigneeMotorola, Inc.
InventorGeorge N. Maracas
Life of this patent
Estimating the term…
Abstract
A method of performing a binding assay which comprises the steps of separating a plurality of sample fragments into a plurality of subsamples and applying each of the plurality of subsamples to a respective one of a plurality of binding assays. A system which performs the aforementioned steps is also disclosed.
Claims22
A method comprising the steps of:
- separating a plurality of sample fragments into a plurality of subsamples including a first subsample and a second subsample;
- applying the first subsample to a first binding assay for detecting any of 4.sup.m different base sequences having a length of m bases;
- and
- applying the second subsample to a second binding assay for detecting any of 4.sup.n different base sequences having a length of n bases.
1 more element
A method comprising the steps of:
- providing a plurality of binding assays including a first binding assay and a second binding assay, the first binding assay for detecting any of 4.sup.m different base sequences having a length of m bases, the second binding assay for detecting any of 4.sup.n different base sequences having a length of n bases;
- generating a first field within a first channel to separate, within the first channel, a plurality of unseparated sample fragments into a plurality of subsamples including a first subsample and a second subsample;
- generating a second field in a second channel in communication with the first channel to isolate the first subsample within the second channel, wherein the second field is generally perpendicular to the first field;
- generating a third field in a third channel in communication with the first channel to isolate the second subsample within the third channel, wherein the third field is generally perpendicular to the first field;
- applying the first subsample isolated in the second channel to the first binding assay;
- and
- applying the second subsample isolated in the third channel to the second binding assay.
4 more elements
A method comprising the steps of:
- providing a plurality of binding assays including a first binding assay and a second binding assay, the first binding assay for detecting any of 4.sup.m different base sequences having a length of m bases, the second binding assay for detecting any of 4.sup.n different base sequences having a length of n bases;
- separating, within a first channel, a plurality of unseparated sample fragments into a plurality of subsamples including a first subsample and a second subsample;
- isolating the first subsample within a second channel in communication with the first channel, wherein the second channel is generally perpendicular to the first channel;
- isolating the second subsample within a third channel in communication with the first channel, wherein the third channel is generally perpendicular to the first channel;
- applying the first subsample isolated in the second channel to the first binding assay;
- and
- applying the second subsample isolated in the third channel to the second binding assay.
4 more elements
A method comprising the steps of:
- providing a plurality of binding assays including a first binding assay and a second binding assay;
- separating, within a first channel, a plurality of unseparated sample fragments into a plurality of subsamples including a first subsample and a second subsample;
- isolating the first subsample within a second channel connected to the first channel, the second channel having an outlet;
- isolating the second subsample within a third channel connected to the first channel, the third channel having an outlet;
- migrating the first subsample to the outlet of the second channel;
- migrating the second subsample to the outlet of the third channel;
- providing an applicator having a plurality of transfer elements including a first transfer element and a second transfer element;
- loading the first subsample from the outlet of the second channel to the first transfer element;
- loading the second subsample from the outlet of the third channel to the second transfer element;
- and
- contacting the applicator to a member which supports the plurality of binding assays to apply the first subsample to the first binding assay and the second subsample to the second binding assay.
8 more elements
A method comprising the steps of:
- separating a plurality of sample fragments into a plurality of subsamples including a first subsample and a second subsample;
- applying the first subsample to a first binding assay for detecting any of 4.sup.m different base sequences having a length of m bases;
- and
- applying the second subsample to a second binding assay for detecting any of 4.sup.n different base sequences having a length of n bases;
- wherein n is greater than m.
2 more elements
A method comprising the steps of:
- separating a plurality of sample fragments into a plurality of subsamples including a first subsample and a second subsample, the second subsample having sample fragments of generally greater length than the first subsample;
- applying the first subsample to a first binding assay for detecting any of 4.sup.m different base sequences having a length of m bases;
- and
- applying the second subsample to a second binding assay for detecting any of 4.sup.n different base sequences having a length of n bases;
- wherein n is greater than m.
2 more elements
Dependencies are inferred from the claim text.