Cytoplasmic male sterile Brassica oleracea plants which contain the polima CMS cytoplasm and are male sterile at high and low temperatures
AssigneeSeminis Vegetable Seeds, Inc.
InventorsAnnemarie Elsenga-Boersma, Fransiscus Gerardus Jacobus Maria Van Den Bosch
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Abstract
The present invention involves cytoplasmic male sterile Brassica oleracea plants that contain polima CMS cytoplasm. The plants remain sterile at high temperatures. The Brassica oleracea plants of the present invention can be prepared by traditional breeding techniques. Different Brassica types can then be produced using these plants by employing traditional breeding techniques or by protoplast fusion.
Claims12
A method for producing cytoplasmic male sterile Brassica oleracea plants which are sterile at high and low temperature and which contain polima CMS cytoplasm, the method comprising the steps of:
- a) crossing Brassica campestris cultivar 87110 having ATCC Accession Number 97246 with Brassica oleracea cultivar 87101 and collecting and seeds resulting from said cross;
- b) regenerating said seeds into Brassica napus plants which contain a haploid set of chromosomes;
- c) doubling the chromosomes of the plants of step b to provide Brassica napus plants containing a diploid set of chromosomes;
- d) crossing Brassica campestris cultivar 87118 having ATCC Accession Number 97247 with Brassica oleracea cultivar 87101 and collecting the seeds resulting from said cross;
- e) regenerating said seeds of step d into Brassica napus plants which contain a haploid set of chromosomes;
- f) doubling the chromosomes of the plants of step e to provide Brassica napus plants containing a diploid set of chromosomes;
- g) crossing the plants of step f with Brassica napus cultivar 87102 which is male sterile and contains polima CMS cytoplasm and collecting the seeds resulting from said cross;
- h) regenerating said seeds of step g into Brassica napus plants containing the polima CMS cytoplasm;
- i) crossing the plants of step c with the plants of step h and collecting the seeds resulting from said cross;
- j) regenerating said seeds of step i into Brassica napus plants which are male sterile and contain the polima CMS cytoplasm;
- k) crossing the plants of step j with a Brassica oleracea cultivar and collecting the seeds resulting from said cross;
- l) removing the embryos from the seeds of step k and regenerating said embryos into F.sub.1 plants which are male sterile and contain the polima CMS cytoplasm;
- m) backcrossing the plants from step l with a Brassica oleracea cultivar and collecting the seeds resulting from said backcrossing;
- n) removing the embryos from the seeds of step m and regenerating said embryos into plants which are male sterile and contain the polima CMS cytoplasm;
- o) backcrossing the plants from step n with a Brassica oleracea and collecting the seeds resulting from said backcrossing;
- and
- p) regenerating said seeds of step o into Brassica oleracea plants which are male sterile at high and low temperatures and contain the polima CMS cytoplasm.
Dependencies are inferred from the claim text.