Method for demonstrating an enzymatic activity of micro-organisms
AssigneeBio Merieux
InventorSylvain Orenga
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Abstract
The method consists in the use of one compound of formula: X--NH--R in which X represents one 5-bromoindole-3-yle group and R represents the acyl radical of one amino acid selected between leucine and alanine, as tracer for demonstrating, by the formation of a colored product, a peptidase activity in a culture of micro-organisms.
Claims8
A microorganism culture medium containing, besides the ingredients necessary for culturing microorganisms, at least one compound of formula:
- X--NH--R
- in which X represents a 5-bromoindol-3-yl group and R represents the acyl residue of an amino acid chosen from leucine and alanine.
A method for demonstrating a peptidase activity in a microorganism culture, wherein a tracer, which makes it possible to demonstrate said peptidase activity by formation of a colored product, is added to a culture medium of microorganisms, said tracer comprising at least one compound of formula:
- X--NH--R
- in which X represents a 5-bromoindol-3-yl group and R represents an acyl residue of an amino acid chosen from leucine and alanine.
Dependencies are inferred from the claim text.