Oligosaccharide derived from an antigenic polysaccharide obtained from a pathogenic agent
AssigneePasteur Merieux Serums et Vaccines
InventorMonique Moreau
Life of this patent
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Abstract
An oligosaccharide derived from an antigen polysaccharide obtained from a pathogenic agent, a method for its preparation, and its use particularly as a vaccinal agent. The oligosaccharide is prepared by oxidation-reduction depolymerization reaction.
Claims53
An oligosaccharide which has preserved the labile functional groups and the structure of the repeating units of a polysaccharide obtained from a pathogenic agent, said polysaccharide being a surface antigen of said pathogenic agent, said polysaccharide having repeating units containing labile functional groups and said oligosaccharide being prepared by (i) subjecting said polysaccharide to an oxidation-reduction depolymerization reaction, and (ii) recovering the resulting oligosaccharide.
A pharmaceutical composition comprising a pharmaceutically acceptable carrier and, as an active ingredient, at least one oligosaccharide which has preserved the labile functional groups and the structure of the repeating units of an antigenic polysaccharide obtained from a pathogenic agent, said oligosaccharide being prepared by (i) subjecting said polysaccharide to an oxidation-reduction depolymerization reaction, and (ii) recovering the resulting oligosaccharide.
A method of vaccinating an individual comprising administering to said individual a composition comprising an oligosaccharide which has preserved the labile functional groups and the structure of the repeating units of an antigenic polysaccharide obtained from a pathogenic agent, said polysaccharide being a surface antigen of said pathogenic agent, said polysaccharide having repeating units containing labile functional groups, wherein said oligosaccharide is prepared, starting from said polysaccharide, by a process comprising (i) subjecting said polysaccharide to an oxidation-reduction depolymerization reaction, and (ii) recovering the resulting oligosaccharide.
A process for obtaining an oligosaccharide by depolymerizing an antigenic polysaccharide having repeating units containing labile functional groups, while preserving said labile functional groups and the structure of said repeating units, said antigenic polysaccharide being obtained from a pathogenic agent, said polysaccharide being a surface antigen of said pathogenic agent, said process comprising
- (i) subjecting said antigenic polysaccharide to an oxidation-reduction depolymerization reaction in an aqueous solution with an oxidation-reduction agent, and
- (ii) recovering the resulting oligosaccharide.
An oligosaccharide which has preserved the labile functional groups and the structure of the repeating units of an antigenic polysaccharide obtained from a pathogenic agent, said polysaccharide being a surface antigen of said pathogenic agent and having repeating units containing labile functional groups and said oligosaccharide being prepared, starting from said polysaccharide, by (i) subjecting said polysaccharide to an oxidation-reduction depolymerization reaction at a pH in the range of 3 to 8.5 and a temperature in the range of 4 to 60.degree. C., and (ii) recovering the resulting oligosaccharide.
A process for obtaining an oligosaccharide by depolymerizing an antigenic polysaccharide having repeating units containing labile functional groups, while preserving said labile functional groups and the structure of said repeating units, said antigenic polysaccharide being obtained from a pathogenic agent, said polysaccharide being a surface antigen of said pathogenic agent, said process comprising
- (i) subjecting said antigenic polysaccharide to an oxidation-reduction depolymerization reaction in an aqueous solution at a pH in the range of 3 to 8.5 and at a temperature in the range of 4 to 60.degree. C., and
- (ii) recovering the resulting polysaccharide.
A process for obtaining an oligosaccharide by depolymerizing an antigenic polysaccharide having repeating units containing labile functional groups, while preserving said labile functional groups and the structure of said repeating units, said antigenic polysaccharide being obtained from a pathogenic agent, said polysaccharide being a surface antigen of said pathogenic agent, said process comprising
- (i) subjecting said antigenic polysaccharide to an oxidation-reduction depolymerization reaction in the presence of ascorbic acid, and
- (ii) recovering the resulting oligosaccharide.
A process for obtaining an oligosaccharide by depolymerizing an antigenic polysaccharide having repeating units containing labile functional groups, while preserving said labile functional groups and the structure of said repeating units, said antigenic polysaccharide being obtained from a pathogenic agent, said polysaccharide being a surface antigen of said pathogenic agent, said process comprising
- (i) subjecting said antigenic polysaccharide to an oxidation-reduction depolymerization reaction in the presence of hydrogen peroxide, and
- (ii) recovering the resulting oligosaccharide.
Dependencies are inferred from the claim text.