Gene for fatty acid desaturase, vector containing said gene, plant transformed with said gene, and process for creating said plant
AssigneeKirin Beer Kabushiki Kaisha
InventorsOsamu Nishizawa, Toshihiro Toguri
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Abstract
Genes encoding proteins having an activity of desaturating lipid-bound fatty acids at the .DELTA.9 position; vectors which contain the genes or polynucleotides containing part of thereof; plant cells transformed with the genes or polynucleotides containing part of thereof; a method for creating plants by regenerating the plant cells to reproduce mature plants; and plants transformed with the genes or polynucleotides containing part of thereof.
Claims9
An isolated polynucleotide molecule encoding a protein having an activity of desaturating lipid-bound fatty acids at the .DELTA.9 position, wherein said polynucleotide molecule comprises a nucleotide sequence that is present in the genus Anacystis and that hybridizes to a probe having a nucleotide the sequence of SEQ ID NO:1 or SEQ ID NO:3 under (A) an incubation condition of 55.degree. C. for 16 hours in a solution consisting of 6.times.SSPE, 02% SDS and 100 g/ml herring sperm DNA, and (B) washing condition of twice for 15 minutes each in 2.times.SSC at room temperature.
A polynucleotide molecule encoding a protein having an activity of desaturating lipid-bound fatty acids at the .DELTA.9 position, wherein said protein comprises the amino acid sequence shown in SEQ ID NO:4.
A polynucleotide molecule encoding a protein having an activity of desaturating lipid-bound fatty acids at the .DELTA.9 position, wherein the polynucleotide molecule comprises the nucleotide sequence of SEQ ID NO:
- 3.
A method for creating a plant cell having an improved chilling-resistance, which comprises introducing a polynucleotide molecule that is present in the genus Anacystis and that encodes a .DELTA.9 desaturase having an activity of desaturating lipid-bound fatty acids at the .DELTA.9 position.
A method for creating a plant having an improved chilling-resistance, which comprises introducing a polynucleotide molecule that is present in the genus Anacystis and that encodes a .DELTA.9 desaturase having an activity of desaturating lipid-bound fatty acids at the .DELTA.9 position.
Dependencies are inferred from the claim text.