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US 6,042,832 AGranted
Polypeptides fused with alfalfa mosaic virus or ilarvirus capsid proteins
AssigneeThomas Jefferson University
InventorsHilary Koprowski, Vidadi Yusibov, Douglas Craig Hooper, Anna Modelska
Life of this patent
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Abstract
A fusion capsid protein comprising a plant virus capsid protein fused to an antigenic polypeptide is used as a molecule for presentation of that polypeptide to the immune system of an animal such as a human. The plant virus capsid protein is that of an alfalfa mosaic virus (AlMV) or ilarvirus.
Claims12
A process of administering a polypeptide to an animal, the process comprising the steps of:
- (1) infecting a plant cell with recombinant plant virus nucleic acid that will be processed in a plant cell to produce a fusion capsid protein, said fusion capsid protein comprising a plant virus capsid protein and the polypeptide, such that the polypeptide is fused to the amino terminus of said plant virus capsid protein, wherein said polypeptide is not a plant virus capsid protein, said plant virus capsid protein being an alfalfa mosaic virus (AlMV) capsid protein or ilarvirus capsid protein, wherein said recombinant plant virus nucleic acid comprises an AlMV or ilarvirus nucleic acid vector and further comprises nucleic acid coding for the polypeptide such that the polypeptide will be fused to the amino-terminus of the AlMV capsid protein or ilarvirus capsid protein, said infecting resulting in an infected cell;
- (2) cultivating the infected cell under conditions where said infected cell makes the fusion capsid protein, and
- (3) administering the fusion capsid protein to an animal, thereby inducing the production of antibodies against at least the polypeptide in said animal.
A process for producing a polypeptide, the process comprising the steps of:
- (1) infecting a plant cell with recombinant plant virus nucleic acid that will be processed by a plant cell to produce a fusion capsid protein, said fusion capsid protein comprising a plant virus capsid protein and the polypeptide such that the polypeptide is fused to the amino terminus of said plant virus capsid protein, said plant virus capsid protein being an AlMV or ilarvirus capsid protein, said polypeptide not being a plant virus capsid protein, said infection resulting in an infected cell;
- (2) cultivating the infected cell under conditions where said infected cell makes the fusion capsid protein.
Dependencies are inferred from the claim text.