US 6,040,160 AGranted

Method of producing L-lysine by fermentation

AssigneeAjinomoto Co., Inc.

InventorsHiroyuki Kojima, Yuri Ogawa, Kazue Kawamura, Konosuke Sano

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Abstract

A bacterium belonging to the genus Escherichia, which is transformed by introducing, into its cells, a DNA coding for a dihydrodipicolinate synthase originating from a bacterium belonging to the genus Escherichia having mutation to desensitize feedback inhibition by L-lysine and a DNA coding for an aspartokinase III originating from a bacterium belonging to the genus Escherichia having mutation to desensitize feedback inhibition by L-lysine; preferably a bacterium belonging to the genus Escherichia in which a dihydrodipicolinate reductase gene and a diaminopimelate dehydrogenase gene originating from Brevibacterium lactofermentum (or a succinyldiaminopimelate transaminase gene and a succinyldiaminopimelate deacylase gene) are further enhanced, is cultivated in an appropriate medium, L-lysine is produced and accumulated in a culture thereof, and L-lysine is collected from the culture.

Claims22

Dependent claims

An isolated DNA coding for a dihydrodipicolinate synthase originating from a bacterium belonging to the genus Escherichia, wherein the dihydrodipicolinate synthase has a mutation which desensitizes feedback inhibition by L-lysine, wherein the mutation is selected from the group consisting of

(a) a mutation to replace the alanine residue at the 81st position as counted from the N-terminal in the amino acid sequence of the dihydrodipicolinate synthase of SEQ ID NO:
4 with another amino acid residue,
(b) a mutation to replace the histidine residue at the 118th position as counted from the N-terminal in the amino acid sequence of the dihydrodipicolinate synthase of SEQ ID NO:
4 with another amino acid residue, and
(c) a mutation to replace the alanine residue at the 81st position as counted from the N-terminal in the amino acid sequence of the dihydrodipicolinate synthase of SEQ ID NO:
4 with another amino acid residue and replace the histidine residue at the 118th position as counted from the N-terminal in the amino acid sequence of the dihydrodipicolinate synthase of SEQ ID NO:
4 with another amino acid residue,
(d) a mutation to replace the alanine residue corresponding to the 81st position as counted from the N-terminal in the amino acid sequence of the dihydrodipicolinate synthase of SEQ ID NO:
4 with another amino acid residue,
(e) a mutation to replace the histidine residue corresponding to the 118th position as counted from the N-terminal in the amino acid sequence of the dihydrodipicolinate synthase of SEQ ID NO:
4 with another amino acid residue, and
(f) a mutation to replace the alanine residue corresponding to the 81st position as counted from the N-terminal in the amino acid sequence of the dihydrodipicolinate synthase of SEQ ID NO:
4 with another amino acid residue and replace the histidine residue corresponding to the 118th position as counted from the N-terminal in the amino acid sequence of the dihydrodipicolinate synthase of SEQ ID NO:
4 with another amino acid residue.

11 more elements

A bacterium belonging the genus Escherichia which is transformed with a DNA coding for a dihydrodipicolinate synthase originating from a bacterium belonging to the genus Escherichia and having mutation to desensitize feedback inhibition by L-lysine, wherein the mutation is selected from the group consisting of

(a) a mutation to replace the alanine residue at the 81st position as counted from the N-terminal in the amino acid sequence of the dihydrodipicolinate synthase of SEQ ID NO:
4 with another amino acid residue,
(b) a mutation to replace the histidine residue at the 118th position as counted from the N-terminal in the amino acid sequence of the dihydrodipicolinate synthase of SEQ ID NO:
4 with another amino acid residue, and
(c) a mutation to replace the alanine residue at the 81st position as counted from the N-terminal in the amino acid sequence of the dihydrodipicolinate synthase of SEQ ID NO:
4 with another amino acid residue and replace the histidine residue at the 118th position as counted from the N-terminal in the amino acid sequence of the dihydrodipicolinate synthase of SEQ ID NO:
4 with another amino acid residue,
(d) a mutation to replace the alanine residue corresponding to the 81st position as counted from the N-terminal in the amino acid sequence of the dihydrodipicolinate synthase of SEQ ID NO:
4 with another amino acid residue,
(e) a mutation to replace the histidine residue corresponding to the 118th position as counted from the N-terminal in the amino acid sequence of the dihydrodipicolinate synthase of SEQ ID NO:
4 with another amino acid residue, and
(f) a mutation to replace the alanine residue corresponding to the 81st position as counted from the N-terminal in the amino acid sequence of the dihydrodipicolinate synthase of SEQ ID NO:
4 with another amino acid residue and replace the histidine residue corresponding to the 118th position as counted from the N-terminal in the amino acid sequence of the dihydrodipicolinate synthase of SEQ ID NO:
4 with another amino acid residue.

11 more elements

Dependencies are inferred from the claim text.