Diagnostic compositions and devices utilizing same
AssigneeMercury Diagnostics, Inc.
InventorsJoel S. Douglas, Karen R. Drexler
Life of this patent
Estimating the term…
Abstract
A dry chemistry dye indicator composition provides improved shelf life, stable color indication end point and capability for a system at near normal pH. The novel dry chemistry dye indication system comprises 3-Methyl-6-(sodium sulfonate)-benzothiazolinone-(2)-hydrazone (MBTH-S). A preferred dye systems are based on the dye couple (MBTH-S) and 8-anilino-1-naphthalenesulfonate (ANS), and the dye couple MBTH-S and N-(3-sulfopropyl)analine. These dye indicator systems are used in conventional blood chemistry test strips and are particularly preferred for indication of glucose in blood.
Claims18
A composition for a dry chemistry reagent indicator comprising:
- 3-Methyl-6-(M sulfonate)-benzothiazolinone-(2)-hydrazone, wherein M is a positive charge ion providing a stable aqueous salt thereof;
- a second dye component selected from the group consisting of 3,3-dimethylaminobenzoic acid, 3,5-dichloro-2-hydroxybenzenesulfonic acid, 8-anilino-1-naphthalenesulfonate and N-(3-sulfopropyl)aniline;
- and
- glucose oxidase.
A device for testing the presence or concentration of an analyte in a fluid sample comprising:
- a support member,
- a composition positioned on or impregnated in said support said composition comprising 3-Methyl-6-(M sulfonate)-benzothiazolinone-(2)-hydrazone, wherein M is a positive charge ion providing a stable aqueous salt thereof, a second dye component selected from the group consisting of 3,3-dimethylaminobenzoic acid, 3,5-dichloro-2-hydroxybenzenesulfonic acid, 8-anilino-1-naphthalenesulfonate and N-(3-sulfopropyl)aniline;
- and glucose oxidase;
- whereby the support member is adapted for receiving a fluid sample such that the fluid sample contacts said composition and adapted such that the support provides for inspection or reading of the color change produced by said composition after contact with the fluid sample.
A method of testing a fluid for the presence or concentration of an analyte comprising:
- applying a fluid sample to a support member having positioned thereon or impregnated therein a composition comprising 3-Methyl-6-(M sulfonate)-benzothiazolinone-(2)-hydrazone, wherein M is a positive charge ion providing a stable aqueous salt thereof, a second dye component selected from the group consisting of 3,3-dimethylaminobenzoic acid, 3,5-dichloro-2-hydroxybenzenesulfonic acid, 8-anilino-1-naphthalenesulfonate and N-(3-sulfopropyl)aniline;
- and glucose oxidase;
- whereby the support member is adapted for receiving a fluid sample which contacts said composition and adapted to provide for inspection or reading of the color change produced by said composition after contact with the fluid sample;
- and
- reading or measuring the color indication provided by said composition after contact with said fluid sample.
Dependencies are inferred from the claim text.