Method for reducing expression variability of transgenes in plant cells

A DNA construct comprising an expression cassette, which construct comprises, in the 5' to 3' direction, a first scs insulator a transcription initiation region, a structural gene positioned downstream from said transcription initiation region and operatively associated therewith, and a sacond scs insulator.

A recombinant plant comprising transformed plant cells, said transformed plant cells containing a heterologous DNA construct comprising an expression cassette, which construct comprises, in the 5' to 3' direction, a first scs insulator, a transcription initiation region, a structural gene positioned downstream from said transcription initiation region and operatively associated therewith, and a second scs insulator.

A DNA construct comprising an expression cassette, which construct comprises, in the 5' to 3' direction, a first insulator, a transcription initiation region, a structural gene positioned downstream from said transcription initiation region and operatively associated therewith, and a second insulator.

A method of making recombinant plant cells having increased expression of foreign DNA therein, said method comprising:

• providing a plurality of plant cells capable of regeneration;
• transforming each of said plant cells with a DNA construct comprising an expression cassette, which construct comprises, in the 5' to 3' direction, a first scs insulator, a transcription initiation region, a structural gene positioned downstream from said transcription initiation region and operatively associated therewith, and a sacond scs insulator;
• wherein expression of the structural gene occurs in more of said plurality of plant cells compared to that which would occur in the absence of said insulator.