Male tissue-preferred regulatory region and method of using same

A method of producing a transformed plant that expresses an exogenous nucleotide sequence in a male tissue-preferred manner comprising introducing into a plant said exogenous nucleotide sequence operably linked to a male tissue preferred regulatory region comprising the nucleotide sequence of SEQ ID NO. 1 or SEQ ID NO. 2 or those nucleotide sequences which hybridize to either of SEQ ID NO. 1 or NO. 2 under conditions of high stringency.

A method of controlling fertility in a plant comprising producing a transformed plant wherein the male tissue-preferred regulatory region of claim1, operably linked to an exogenous gene, expresses said exogenous nucleotide sequence such that fertility is impacted.

The method according to claim3 wherein said exogenous nucleotide sequence encodes a cytotoxic molecule.

A method of producing hybrid seed, comprising planting in cross pollinating juxtaposition, a first male fertile plant and a second male infertile plant, the second male infertile plant produced by introducing into said second plant an exogenous nucleotide sequence operably linked to a male tissue-preferred regulatory promoter comprising the nucleotide sequence of SEQ ID NO. 1 or SEQ ID NO. 2 or those nucleotide sequences which hybridize to either of SEQ ID NO. 1 or NO. 2 under conditions of high stringency such that said second plant is rendered male infertile, allowing said cross pollination to occur and harvesting the resulting seed.

A transformed plant expressing an exogenous nucleotide sequence in a male tissue-preferred manner comprising an exogenous nucleotide sequence operably linked to a regulatory region comprising a nucleotide sequence of SEQ ID NO. 1 or SEQ ID NO. 2 or those nucleotide sequences which hybridize to either of SEQ ID NO. 1 or NO. 2 under conditions of high stringency.