Detection of ligand Interaction with polymeric material
AssigneeNew York University
InventorsVictor Morozov, Tamara Morozova
Life of this patent
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Abstract
A method and apparatus for measuring ligand binding to DNA or protein uses changes in the mechanical properties of strips of the DNA or protein to detect the formation of ligand complexes. Samples strips are prepared by pouring protein solution onto a glass surface, drying the solution, and causing the protein to be cross-linked; the cross-linked film is scraped off, cut into strips, and mounted in the stress/strain measuring apparatus, which includes force and strain transducers. The sample is held by arms and can be immersed into solutions. The arms are attached to transducers. The apparatus includes a sample holder. The sample films may include end reinforcements and may be pinned or glued to the arms.
Claims44
An apparatus for measurement of the effect of chemicals on a sample film of polymeric material, comprising:
- a pair of supporting members, each with a sample-holding tip at one end thereof constructed and adapted to be capable of holding an end of a sample film of polymeric material having a thickness on the order of a micron;
- force means, attached to at least one of said supporting members, for applying force to the sample film held by said tips when in use;
- and
- measurement means, attached directly or indirectly to the sample film, for measuring changes in a property of the sample film upon being subjected to a chemical when in use.
An apparatus for measurement of the effect of chemicals on a sample film of polymeric material, comprising:
- a pair of supporting members, each with a sample-holding tip at one end thereof, for holding an end of the sample film of polymeric material, wherein both of said sample holding tips anchor the sample film simultaneously;
- force means, attached to at least one of said supporting members, for applying force to the sample film held by said tips when in use;
- and
- measurement means, attached directly or indirectly to the sample film, for measuring changes in a property of the sample film upon being subjected to a chemical when in use.
Dependencies are inferred from the claim text.