Recombinant infectious non-segmented negative strand RNA virus
OwnerKarl Klaus Conzelmanninventor
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Abstract
The present invention provides the generation of infectious replicating non-segmented negative-stranded RNA virus, entirely from cloned cDNA. This process offers the possibility to introduce mutations into the virus genome by means of recombinant DNA techniques.
Claims19
A genetically manipulated, infectious, replicating, non-segmented negative-stranded RNA virus mutant, comprising at least one alteration selected from an insertion and a deletion, wherein the alteration is in a region of the virus genome selected from an open reading frame, a pseudogene region and an intergenic region.
A process for the preparation of an infectious, replicating, non-segmented, negative-stranded RNA virus comprising the steps of:
- a) providing a host cell expressing a heterologous RNA polymerase;
- b) introducing into said host cell
- 1) one or more DNA molecules encoding the virus N, P and L proteins, or analogs thereof operably linked to expression control sequence functional in said host cell;
- and
- 2) a DNA molecule comprising the cDNA of the non-segmented negative-stranded RNA virus wherein the cDNA encodes the entire genome of the virus or is modified by the incorporation of a mutation, and wherein the DNA molecule is transcribed by the heterologous RNA polymerase, and
- c) isolating the viruses produced by the cells.
Dependencies are inferred from the claim text.