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AssigneeThe Perkin-Elmer Corporation
InventorsLawrence A. Haff, Enrico Picozza, Will Bloch, Robert Ragusa, Joseph DiCesare, David Tracy, Paul Saviano, Timothy M. Woudenberg, Richard W. Noreiks
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Apparatus and method for performing a nucleic acid amplification reaction and preferably a polymerase chain reaction (PCR) in a reaction mixture in at least one capillary tube. Several different embodiments are disclosed. One embodiment cycles a sample through a capillary tube loop passing through two thermostatted fluid baths. Another embodiment has the capillary tube routed alternatingly between two heat exchangers to that the sample makes only one pass through the tube. Other embodiments maintain the heat exchangers stationary and translate the samples between them. Still further embodiments maintain the samples stationary and either automatically translate or rotate the heat exchangers past the samples contained within the capillary tubes to perform the thermal cycles necessary for the amplification reaction.
A method for performing a nucleic acid amplification reaction including denaturation, annealing, and extension processes, simultaneously in a plurality of reaction mixtures contained in a plurality of capillary tubes, each mixture containing a target nucleic acid to be amplified, comprising the steps of:
A method of amplifying, by a nucleic acid amplification reaction, at least one target nucleic acid sequence in each one of a plurality of reaction mixtures, the PCR including denaturation, annealing, and extension processes, the method comprising the steps of:
A method of amplifying, by a nucleic acid amplification reaction, at least one target nucleic acid sequence in each one of a plurality of reaction mixtures, the amplification reaction including denaturation, annealing, and extension processes, the method comprising the steps of:
A method of automatically performing a nucleic acid amplification reaction in a reaction mixture in a capillary tube containing a target nucleic acid segment to be amplified, the amplification reaction including denaturation, annealing and extension processes, the method comprising the steps of:
Dependencies are inferred from the claim text.