Fructosyl amino acid oxidase, process for producing the same, and method of assaying amadori compounds using the enzyme
AssigneeKyoto Daiichi Kagaku Co., Ltd.
InventorsNobuo Kato, Yasuyoshi Sakai, Yoshiki Tani, Hiroshi Fukuya, Masayuki Yagi, Toshikatsu Sakai, Kaori Ishimaru, Satoshi Cyousa
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Abstract
The present invention relates to a fructosyl amino acid oxidase that is obtainable from a culture of a strain of the genus Aspergillus. This strain grows in a selective medium containing at least either of fructosyl lysine and fructosyl-N.sup..alpha. -Z-lysine and is capable of producing a fructosyl amino acid oxidase.
Claims5
A fructosyl amino acid oxidase from a culture of a strain of the genus Aspergillus, which has the following physicochemical characteristics:
- a) it catalyzes oxidation of an amadori campound in the presence of oxygen to generate .alpha.-ketoaldehyde, amine derivative and hydrogen peroxide;
- b) it is active on fructosyl lysine and fructosyl valine as a substrate, wherein the substrate specificity on fructosyl lysine is higher than on fructosyl valine;
- c) the optimum pH thereof is 8.5;
- and
- d) it is inhibited by manganese, cobalt, zinc or mercury ion.
A fructosyl amino acid oxidase from a culture of a strain of the genus Aspergillus, which has the following physicochemical characteristics:
- a) it catalyzes oxidation of an amadori campound in the presence of oxygen to generate .alpha.-ketoaldehyde, amine derivative and hydrogen peroxide;
- b) it is active on fructosyl lysine and fructosyl valine as a substrate, wherein the substrate specificity on fructosyl lysine is higher than on fructosyl valine;
- c) the molecular weight thereof is, when determined by gel filtration on Superdex 200 pg, about 94 kD;
- and
- d) it is inhibited by copper, zinc, silver or mercury ion.
Dependencies are inferred from the claim text.