Diagnosis, prevention and treatment of ulcerative colitis, and clinical subtypes thereof, using microbial UC pANCA antigens
AssigneeThe Regents of the University of California
InventorsJonathan Braun, Offer Cohavy
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Abstract
The present invention relates to microbial UC pANCA antigens. The invention provides methods of diagnosing ulcerative colitis (UC) and methods of inducing tolerance in a pANCA-positive patient with UC using a histone H1-like antigen. The invention further provides methods of diagnosing UC and methods of inducing tolerance in a pANCA-positive patient with UC using a porin antigen. Methods of diagnosing UC and methods of inducing tolerance in a pANCA-positive patient with UC using a Bacteroides antigen also are provided.
Claims15
A method of diagnosing ulcerative colitis (UC) in a subject suspected of having inflammatory bowel disease, comprising:
- (a) obtaining a sample from said subject;
- (b) contacting said sample with a porin antigen having an amino acid sequence selected from the group consisting of SEQ ID NOS:
- 28 to 30, or a modification or fragment thereof having immunological reactivity with pANCA, under conditions suitable to form a complex of said porin antigen, or modification or fragment thereof, with antibody to said porin antigen;
- and
- (c) detecting the presence or absence of said complex, wherein the presence of said complex indicates that said subject has UC.
A method of diagnosing a perinuclear anti-neutrophil cytoplasmic antibody-positive (pANCA-positive) clinical subtype of UC in a patient with UC, comprising:
- (a) obtaining a sample from said patient with UC;
- (b) contacting said sample with a porin antigen having an amino acid sequence selected from the group consisting of SEQ ID NOS:
- 28 to 30, or a modification or fragment thereof having immunological reactivity with pANCA, under conditions suitable to form a complex of said porin antigen, or modification or fragment thereof, with antibody to said porin antigen;
- and
- (c) detecting the presence or absence of said complex, wherein the presence of said complex indicates that said patient has said pANCA-positive clinical subtype of UC.
A method of determining susceptibility to UC in an individual, comprising:
- (a) obtaining a sample from said individual;
- (b) contacting said sample with a porin antigen having an amino acid sequence selected from the group consisting of SEQ ID NOS:
- 28 to 30, or a modification or fragment thereof having immunological reactivity with pANCA, under conditions suitable to form a complex of said porin antigen, or modification or fragment thereof, with antibody to said porin antigen;
- and
- (c) detecting the presence or absence of said complex, wherein the presence of said complex indicates that said individual has increased susceptibility to UC.
Dependencies are inferred from the claim text.