Method for covalent attachment of biomolecules to surfaces of medical devices

A method of forming a coating on a surface of a medical device, the coating imparting improved biocompatibility characteristics to the surface, the surface being suitable for contacting bodily tissues or fluids in or temporarily removed from a living mammalian subject, the method comprising:

• (a) providing the medical device, the device having a suitable biomaterial forming the surface, a 1,2 dicarbonyl moiety being disposed on the surface;
• (b) providing a biomolecule, the biomolecule comprising a guanidino moiety;
• and
• (c) combining the guanidino moiety with the 1,2 dicarbonyl moiety to form covalent bonds, the covalent bonds immobilizing the biomolecule on the surface, the immobilized biomolecule forming the coating.

A method of forming a coating on a surface of a medical device, the coating imparting improved biocompatibility characteristics to the surface, the surface being suitable for contacting bodily tissues or fluids in or temporarily removed from a living mammalian subject, the method comprising:

• (a) providing the medical device, the device having a suitable biomaterial forming the surface, an amine moiety being disposed on the surface;
• (b) combining the amine moiety with a guanidino forming agent to form a guanidino moiety on the surface;
• (c) providing a biomolecule, the biomolecule comprising a 1,2 dicarbonyl moiety;
• and
• (d) combining the 1,2 dicarbonyl moiety with the guanidino moiety to form covalent bonds, the covalent bonds immobilizing the biomolecule on the surface, the immobilized biomolecule forming the coating.

A method of forming a coating on a surface of a medical device, the coating imparting improved biocompatibility characteristics to the surface, the surface being suitable for contacting bodily tissues or fluids in or temporarily removed from a living mammalian subject, the method comprising:

• (a) providing the medical device, the device having a suitable biomaterial forming the surface, a 1,2 dicarbonyl moiety being disposed on the surface;
• (b) providing a biomolecule, the biomolecule comprising an amine moiety;
• (c) combining the amine moiety with a guanidino forming agent to form a guanidino moiety;
• and
• (d) combining the guanidino moiety with the 1,2 dicarbonyl moiety to form covalent bonds, the covalent bonds immobilizing the biomolecule on the surface, the immobilized biomolecule forming the coating.

A method of forming a crosslinked coating on a surface of a medical device, the coating being suitable for contacting bodily tissues or fluids in or temporarily removed from a living mammalian subject, the method comprising:

• (a) providing a first biomolecule, the first biomolecule comprising a 1,2 dicarbonyl moiety;
• (b) providing a second biomolecule, the second biomolecule comprising a guanidino moiety;
• (c) combining the guanidino moiety with the 1,2 dicarbonyl moiety to form covalent bonds, the covalent bonds crosslinking the two materials together to form a crosslinked biomaterial;
• (d) providing a medical device surface:
• and
• (e) immobilizing the crosslinked biomaterial on the surface, the crosslinked biomaterial forming the coating.

A method of forming a crosslinked coating on a surface of a medical device, the coating imparting improved biocompatibility characteristics to the surface, the surface being suitable for contacting bodily tissues or fluids in or temporarily removed from a living mammalian subject, the method comprising:

• (a) providing the medical device, the device having a suitable biomaterial forming the surface, a first guanidino moiety being disposed on the surface;
• (b) providing two or more biomolecules, the biomolecules comprising a second guanidino moiety and a 1,2 dicarbonyl moiety;
• (c) combining the biomolecules with the surface;
• and
• (d) allowing the first and second guanidino moieties to combine with the 1,2 dicarbonyl moieties to form covalent bonds, the bonds immobilizing and crosslinking the biomolecules on the surface, the immobilized and crosslinked biomolecules forming the coating.

A method of forming a crosslinked coating on a surface of a medical device, the coating imparting improved biocompatibility characteristics to the surface, the surface being suitable for contacting bodily tissues or fluids in or temporarily removed from a living mammalian subject, the method comprising:

• (a) providing the medical device, the device having a suitable biomaterial forming the surface, a first 1,2 dicarbonyl moiety being disposed on the surface;
• (b) providing two or more biomolecules, the biomolecules comprising a guanidino moiety and a second 1,2 dicarbonyl moiety;
• (c) combining the biomolecules with the surface;
• and
• (d) allowing the guanidino moieties to combine with the first and second 1,2 dicarbonyl moieties to form covalent bonds, the bonds immobilizing and crosslinking the biomolecules on the surface, the immobilized and crosslinked biomolecules forming the coating.