Vitro maintenance of hematopoietic stem cells
AssigneeOsiris Therapeutics, Inc.
InventorsMark A. Thiede, Mark F. Pittenger, Gabriel Mbalaviele
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Abstract
The present invention is directed to human mesenchymal stem cells isolated from a tissue specimen, such as marrow cells, and to the method of co-culturing isolated mesenchymal stem cells and/or mesenchymal stem cell-derived adipocytes with hematopoietic progenitor cells such that the hematopoietic stem cells retain their phenotype.
Claims25
A method of maintaining human hematopoietic stem cells in vitro comprising co-culturing human mesenchymal stem cells with the hematopoietic stem cells such that at least some of the hematopoietic stem cells maintain their stem cell phenotype.
A method of maintaining human hematopoietic stem cells in vitro comprising co-culturing adipocytes with the hematopoietic stem cells such that at least some of the hematopoietic stem cells maintain their stem cell phenotype.
A composition for maintaining human hematopoietic stem cells in vitro such that at least some of the hematopoietic stem cells retain their stem cell phenotype, comprising human hematopoietic stem cells and human mesenchymal stem cells.
A composition for maintaining human hematopoietic stem cells in vitro such that at least some of the hematopoietic stem cells retain the stem cell phenotype, comprising human hematopoietic stem cells and adipocytes.
Dependencies are inferred from the claim text.