Therapeutic and prophylactic methods using heat shock proteins

A composition comprising an immunogenic amount of a complex of a heat shock protein noncovalently bound to an antigenic peptide or protein, wherein the complex is made in vitro, and wherein the antigenic peptide or protein is not isolated from a heat shock protein-peptide complex from within a cell, and wherein the antigenic peptide or protein is an antigen of a virus or antigenic fragment or antigenic derivative thereof, said virus being selected from the group consisting of hepatitis type B, hepatitis type C, varicella, adenovirus, herpes simplex type I (HSV-I), herpes simplex type II (HSV-II), rinderpest, rhinovirus, echovirus, rotavirus, respiratory syncytial virus, papilloma virus, papova virus, cytomegalovirus, echinovirus, arbovirus, huntavirus, coxsackie virus, mumps virus, measles virus, rubella virus, polio virus, human immunodeficiency virus type I (HIV-I), and human immunodeficiency virus type II (HIV-II).

A composition comprising an immunogenic amount of a complex of a heat shock protein noncovalently bound to an antigenic peptide or protein, wherein the complex is made in vitro, and wherein the antigenic peptide or protein is not isolated from a heat shock protein-peptide complex from within a cell, and wherein the antigenic peptide or protein is an antigen of a bacterium or antigenic fragment or antigenic derivative thereof.

A composition comprising an immunogenic amount of a complex of a heat shock protein noncovalently bound to an antigenic peptide or protein, wherein the complex is made in vitro, and wherein the antigenic peptide or protein is not isolated from a heat shock protein-peptide complex from within a cell, and wherein the antigenic peptide or protein is an antigen of a fungus or antigenic fragment or antigenic derivative thereof.

A composition comprising an immunogenic amount of a complex of a heat shock protein noncovalently bound to an antigenic peptide or protein, wherein the complex is made in vitro, and wherein the antigenic peptide or protein is not isolated from a heat shock protein-peptide complex from within a cell, and wherein the antigenic peptide or protein is an antigen of a parasite or antigenic fragment or antigenic derivative thereof.

A composition comprising an immunogenic amount of a complex of a heat shock protein noncovalently bound to an antigenic peptide or protein, wherein the complex is made in vitro, and wherein the antigenic peptide or protein is not isolated from a heat shock protein-peptide complex from within a cell, and wherein the antigenic peptide or protein is an antigen of a protozoa or antigenic fragment or antigenic derivative thereof.

A method of eliciting an immune response in an individual comprising administering to the individual a complex of a heat shock protein noncovalently bound to an antigenic peptide or protein, wherein the complex is made in vitro, and wherein the antigenic peptide or protein is not isolated from a heat shock protein-peptide complex from within a cell and, wherein the antigenic peptide or protein is not isolated from an MHC-peptide complex.

A method of eliciting an immune response in an individual comprising administering to the individual a complex of a heat shock protein noncovalently bound to an antigenic peptide or protein, wherein the complex is made in vitro, and wherein the antigenic peptide or protein is not isolated from a heat shock protein-peptide complex from within a cell and, wherein the antigenic peptide or protein is an antigen of a cancer cell or an antigenic fragment or antigenic derivative thereof.

A method of eliciting an immune response in an individual comprising administering to the individual a complex of a heat shock protein noncovalently bound to an antigenic peptide or protein, wherein the complex is made in vitro, and wherein the antigenic peptide or protein is not isolated from a heat shock protein-peptide complex from within a cell and, wherein the antigenic peptide or protein is an antigen of a pathogen or an antigenic fragment or antigenic derivative thereof, and wherein the pathogen is not an intracellular pathogen.

A kit comprising in a container a purified complex of a heat shock protein noncovalently bound to an antigenic peptide or protein in a pharmaceutically acceptable carrier, wherein the complex is made in vitro, and wherein the antigenic peptide or protein is not isolated from a heat shock protein-peptide complex from within a cell, and wherein the antigenic peptide or protein is an antigen of a cancer cell or an antigenic fragment of antigenic derivative thereof.

A kit comprising in a container a purified complex of a heat shock protein noncovalently bound to an antigenic peptide or protein in a pharmaceutically acceptable carrier, wherein the complex is made in vitro, and wherein the antigenic peptide or protein is not isolated from a heat shock protein-peptide complex from within a cell, and wherein the antigenic molecule is an antigen of a virus selected from the group consisting of hepatitis type B, hepatitis type C, varicella, adenovirus, herpes simplex type I (HSV-I), herpes simplex type II (HSV-II), rinderpest, rhinovirus, echovirus, rotavirus, respiratory syncytial virus, papilloma virus, papova virus, cytomegalovirus, echinovirus, arbovirus, huntavirus, coxsackie virus, mumps virus, measles virus, rubella virus, polio virus, human immunodeficiency virus type I (HIV-I), and human immunodeficiency virus type II (HIV-II).

A kit comprising in a container a purified complex of a heat shock protein noncovalently bound to an antigenic peptide or protein in a pharmaceutically acceptable carrier, wherein the complex is made in vitro, and wherein the antigenic peptide or protein is not isolated from a heat shock protein-peptide complex from within a cell, and wherein the antigenic molecule is an antigen of a pathogen or an antigenic fragment or antigenic derivative thereof, said pathogen being selected from the group consisting of bacterium, fungus, parasite, or protozoa.

A composition comprising an immunogenic amount of a complex of a heat shock protein noncovalently bound to an antigenic peptide or protein, wherein the complex is made in vitro, and wherein the antigenic peptide or protein is not isolated from a heat shock protein-peptide complex from within a cell and, wherein the antigenic peptide or protein is an antigen of a pathogen or an antigenic fragment or antigenic derivative thereof, and wherein the pathogen is not an intracellular pathogen.