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US 6,028,183 AGranted
Pyrimidine derivatives and oligonucleotides containing same
AssigneeGilead Sciences, Inc.
InventorsKuei-Ying Lin, Mark D. Matteucci
Life of this patent
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Abstract
Compounds having structure (1) ##STR1## wherein R.sup.1 is --H a protecting group, a linker or a binding partner; and R.sup.2 and R.sup.34 are as defined in the specification. The invention also provides intermediates and methods make the structure (1) compounds, as well as methods to use the compounds as labels in diagnostic assays and to enhance binding to complementary bases.
Claims30
A compound having the structure (1):
- ##STR38## and tautomers, solvates and salts thereof, wherein R.sup.1 is an oligonucleotide, a protecting group, a linker or --H;
- R.sup.2 is A(Z).sub.X1, wherein A is a spacer and Z independently is a label bonding group optionally bonded to a detectable label, but R.sup.2 is not amine, protected amine, nitro or cyano;
- R.sup.27 is independently --CH.dbd., --N.dbd., --C(C.sub.1 -C.sub.8 alkyl).dbd. or --C(halogen).dbd., but no adjacent R.sup.27 are both --N.dbd., or two adjacent R.sup.27 are taken together to form a ring having the structure, ##STR39## where R.sup.a is independently --CH.dbd., --N.dbd., --C(C.sub.1-8 alkyl).dbd. or --C(halogen).dbd., but no adjacent R.sup.a are both --N.dbd.;
- R.sup.34 is --O--, --S-- or --N(CH.sub.3)--;
- and
- X1 is 1, 2 or 3.
3 more elements
Dependencies are inferred from the claim text.