Recombinant thermostable enzyme which releases trehalose from non-reducing saccharide
AssigneeKabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo
InventorsHitoshi Mitsuzumi, Michio Kubota, Toshiyuki Sugimoto
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Abstract
Disclosed is a recombinant thermostable enzyme which has a molecular weight of about 54,000-64,000 daltons and a pI of about 5.6-6.6, and releases trehalose from non-reducing saccharides having a trehalose structure as an end unit and a degree of glucose polymerization of at least 3. The enzyme has a satisfactorily-high thermostability, i.e. it is not substantially inactivated even when incubated in an aqueous solution (pH 7.0) at 85.degree. C. for 60 min, and this facilitates the production of trehalose on an industrial scale and in a satisfactorily-high yield.
Claims13
An isolated DNA molecule encoding a thermostable enzyme obtainable from a microorganism of the genus Sulfolobus having the following physico-chemical properties:
- (1) Action
- Releasing trehalose from non-reducing saccharides having a trehalose structure as an end unit and a degree of glucose polymerization of at least 3, but not substantially acting on maltooligosaccharides having a degree of glucose polymerization of at least 3;
- (2) Molecular weight
- About 54,000-64,000 daltons on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDA-PAGE);
- (3) Isoelectric point (pI)
- About 5.6-6.6 on isoelectrophoresis;
- and
- (4) Thermostability
- Substantially not inactivated even when incubated in an aqueous solution (pH 7.0) at 85.degree. C. for 60 min.
Dependencies are inferred from the claim text.