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AssigneeUnilever Patent Holdings B.V.
InventorsFranciscus M Klis, Maarten P Schreuder, Holger Y Toschka, Cornelis T Verrips
Estimating the term…
A method is provided for immobilizing an enzyme, comprising immobilizing the enzyme or a functional part thereof to the cell wall of a microbial cell using recombinant DNA techniques. The enzyme is immobilized by linking it to the C-terminal part of a protein that ensures anchoring in the cell wall. Also provided is a recombinant polynucleotide comprising a structural gene encoding an enzyme protein, a part of a gene encoding the C-terminal part of a protein capable of anchoring in a eukaryotic or prokaryotic cell wall, as well as a signal sequence, in addition to a chimeric protein encoded by the recombinant polynucleotide and a vector and a microorganism containing the polynucleotide. The microorganism is suitable for carrying out enzymatic processes on an industrial scale.
A method for immobilizing an enzyme comprising recombinanltly producing an enzyme or a functional fragment thereof linked to the exterior of a host cell, said method comprising localizing the enzyme or functional fragment thereof at the exterior of the cell wall of a fungus by linking the enzyme or the functional part thereof to the anchoring part of a cell wall anchoring protein, which anchoring part is derivable from the C-terrmal part of said anchoring protein.
A fungus containing an expressible first polynucleotide comprising a structural gene encoding a protein providing catalytic activity and at least a part of a gene encoding at least a C-terminal portion of an anchoring protein capable of anchoring in the cell wall of said fungus, said part encoding ar least the anchoring part of said anchoring protein, said first polynucleotide being present either in a vector or in the chromosome of said fungus.
Dependencies are inferred from the claim text.