Fluorescent cyanine dyes
AssigneeLI-COR, Inc.
InventorsGarrick Murray Little, Ramesh Raghavachari, Narasimhachari Narayanan, Harry Leonard Osterman
Life of this patent
Estimating the term…
Abstract
New fluorescent cyanine dyes are useful as reporter groups for labeling biomolecules.
Claims17
A fluorescent dye having the general formula:
- ##STR7## wherein R is --NCS, and x and y, independently, are integers between 1 and about 10.
A dye-labeled nucleotide analog comprising the general structure ##STR8## wherein each of x and y, independently, is an integer between 1 and 10 and each M, independently, is selected from Li, Na, K, NH.sub.4, (CH.sub.3).sub.3 NH, (CH.sub.3 CH.sub.2).sub.3 NH, (CH.sub.3 CH.sub.2).sub.4 N or (CH.sub.3).sub.4 N.
A dye-labeled nucleotide analog comprising the general structure ##STR9## wherein each of x and y, independently, is an integer between 1 and 10 and each M, independently, is selected from Li, Na, K, NH.sub.4, (CH.sub.3).sub.3 NH, (CH.sub.3 CH.sub.2).sub.3 NH, (CH.sub.3 CH.sub.2).sub.4 N or (CH.sub.3).sub.4 N.
A fluorescent dye labeled DNA chain terminator having the general structure:
- ##STR10## wherein each of x and y, independently, is an integer selected from 1 to about 10.
A fluorescent dye labeled DNA chain terminator having the general structure:
- ##STR11## wherein each of x and y, independently, is an integer selected from 1 to about 10.
A fluorescent dye labeled DNA chain terminator having the general structure:
- ##STR12## wherein each of x and y, independently, is an integer selected from 1 to about 10.
A fluorescent dye labeled DNA chain terminator having the general structure:
- ##STR13## wherein each of x and y, independently, is an integer selected from 1 to about 10.
N-(6-hydroxyhexyl)-1,1,2-trimethyl-1H-benz(e)indolinium halide.
N-(6-hydroxyhexyl)-1,1,2-trimethyl-1H-benz(e)indolinium bromide.
A phosphoramidite derivative of a dye having the general formula ##STR14## wherein R is --O--phosphoramidite and x and y, independently, are integers between 1 and about 10.
A nucleotide analog labelled with a dye of the general formula:
- ##STR15## wherein R is --OH, --CO.sub.2 H, NH.sub.2 or --NCS, and x and y, independently, are integers between 1 and about 10.
A DNA chain terminator labelled with a dye of the general formula:
- ##STR16## wherein R is --OH, --CO.sub.2 H, --NH.sub.2 or --NCS and x and y, independently, are integers between 1 and about 10.
A dye phosphoramidite having the formula. ##STR17##
Dependencies are inferred from the claim text.