Self-assembled metal colloid monolayers and detection methods therewith
AssigneeThe Penn State Research Foundation
InventorMichael J. Natan
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Abstract
A biosensor based on complexes between biomolecule receptors and colloidal Au nanoparticles, and more specifically, colloid layers of receptor/Au complexes that can be used to detect biomolecule analytes through measuring of binding-induced changes in electrical resistance or surface plasmon resonance. Also disclosed is a method for detecting and analysing carrier-borne chemical compounds with Raman spectroscopy using an improved SERS substrate. Further disclosed is an improved method for detecting compounds in solvents using capillary electrophoresis in conjunction with Raman spectroscopy.
Claims32
A process for detecting and quantifying an amount of a chemical compound in a carrier, said process comprising:
- placing a sample comprising said chemical compound in said carrier on a surface-enhanced Raman scattering (SERS)-active surface;
- subjecting said sample to Raman spectroscopy;
- and
- analyzing data generated by said Raman spectroscopy and determining a concentration of said compound in said sample from said data
- wherein said SERS-active surface is prepared by a method comprising the steps of:
- coating a substrate with a bifunctional organic film to impart to said substrate a functionality that allows for bonding of metal colloid particles;
- and
- contacting the organic film coated substrate with a solution of colloid metal particles to bind said particles to functional groups on said organic film thereby forming a monolayer or submonolayer of said particles.
A process for detecting and quantifying an amount of a chemical compound in a carrier, said process comprising:
- placing a sample comprising said chemical compound in said carrier on a surface-enhanced Raman scattering (SERS)-active surface;
- subjecting said sample to Raman spectroscopy;
- and
- analyzing data generated by said Raman spectroscopy and determining a concentration of said compound in said sample from said data
- wherein said SERS-active surface is prepared by a method comprising the steps of:
- coating a substrate selected from the group consisting of glass, quartz, alumina, tin oxides and metals, with a bifunctional organic film selected from the group consisting of organosilanes, poly(allylamine) hydrochloride and biotin, to impart to said substrate a functionality that allows for bonding of metal colloid particles, to form an organic film coated substrate;
- and
- immersing the organic film coated substrate in a solution of colloid metal particles to bind said particles to functional groups on said organic film thereby forming a monolayer or submonolayer of said particles.
An improved method for detecting low concentrations of at least one compound in a solvent using capillary electrophoresis, said method comprising:
- providing a surface enhanced Raman scattering (SERS)-active surface on a substrate;
- placing a first end of a capillary tube in a solvent reservoir containing said solvent and placing a second end of said capillary tube on said SERS-active surface;
- applying an electric current between said solvent reservoir and said SERS-active surface to cause said solvent to elute through said capillary tube onto said SERS-active surface;
- moving said second end of said capillary tube across said SERS-active surface whereby different compounds in said solvent are deposited at different positions on said SERS-active surface;
- subjecting said different compounds to Raman spectroscopy;
- and
- analyzing data generated by said Raman spectroscopy and determining a composition of said different compounds in said solvent;
- wherein said SERS-active surface is prepared by a method comprising the steps of:
- coating a substrate with a bifunctional organic film to impart to said substrate a functionality that allows for bonding of metal colloid particles;
- and
- contacting the organic film coated substrate with a solution of colloid metal particles to bind said particles to functional groups on said organic film thereby forming a monolayer or submonolayer of said particles.
Dependencies are inferred from the claim text.