Method for producing a virus from an african green monkey kidney cell line
AssigneesDyncorp, National Institutes of Health
InventorsLouis Potash, Robert M. Chanock, Robert H. Purcell, Albert Z. Kapikian
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Abstract
A method for producing novel African Green Monkey Kidney (AGMK) cell lines is taught. These cell lines which are free of viable adventitious microbial agents are useful as substrates for viruses and for the preparation of viral vaccines.
Claims8
A method for producing a virus selected from the group consisting of rotaviruses, enteroviruses, polioviruses, respiratory viruses, astroviruses, and hepatitis A viruses, using a cell line originating from the kidney of an African Green Monkey, wherein said cell line supports the growth of rotaviruses, enteroviruses, polioviruses, respiratory viruses, astroviruses, and hepatitis A viruses, and wherein said cell line is substantially free of viable adventitious microbial agents, said method comprising the steps of:
- 1. inoculating said cell line with a sample containing an inoculum of said virus;
- 2. incubating said inoculated cell line to permit proliferation of said virus;
- and
- 3. harvesting the virus resulting from step 2.
Dependencies are inferred from the claim text.