Equine herpesvirus-4TK mutant
AssigneesThe University Court of the University of Glasgow, Equine Virology Research Foundation
InventorsDavid Edward Onions, Lesley Nicolson
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Abstract
An Equine herpesvirus-4 (EHV-4) mutant which does not produce a functional thymidine kinase due to a deletion and/or insertion in the gene encoding thymidine kinase, the deletion and/or insertion made at a position within the thymidine kinase gene such as to not substantially alter expression of the UL24 gene. Recombinant DNA comprising DNA of the EHV-4 mutant, host cells containing the recombinant DNA, and a process for the preparation of the EHV-4 mutant.
Claims15
An EHV-4 mutant characterized in that it does not produce a functional thymidine kinase as a result of a deletion and/or insertion in the gene encoding thymidine kinase, the deletion and/or insertion being made at a position within the thymidine kinase gene so as to not alter expression of the UL24 gene such that virus replication is not disrupted;
- wherein the deletion or insertion is in the C-terminal coding region of the thymidine kinase gene.
An EHV-4 mutant characterized in that it does not produce a functional thymidine kinase as a result of a deletion and/or insertion in the gene encoding thymidine kinase, the deletion and/or insertion being made at a position within the thymidine kinase gene so as to not alter expression of the UL24 gene such that virus replication is not disrupted;
- wherein the thymidine kinase gene prior to said deletion and/or insertion has a sequence according to SEQ ID NO:1.
An EHV-4 mutant, characterized in that it does not produce a functional thymidine kinase as a result of a deletion and/or insertion in the gene encoding thymidine kinase, the deletion and/or insertion being made at a position within the thymidine kinase gene such that virus replication is not disrupted;
- wherein the deletion or insertion is in the C-terminal coding region of the thymidine kinase gene.
Dependencies are inferred from the claim text.