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AssigneesChromos Molecular Systems, Inc., The Biological Research Center of the Hungarian Academy of Sciences
InventorsGyula Hadlaczky, Aladar A. Szalay
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Methods for preparing cell lines that contain artificial chromosomes, methods for preparation of artificial chromosomes, methods for purification of artificial chromosomes, methods for targeted insertion of heterologous DNA into artificial chromosomes, and methods for delivery of the chromosomes to selected cells and tissues are provided. Also provided are cell lines for use in the methods, and cell lines and chromosomes produced by the methods. In particular, satellite artificial chromosomes that, except for inserted heterologous DNA, are substantially composed of heterochromatin are provided. Methods for use of the artificial chromosomes, including for gene therapy, production of gene products and production of transgenic plants and animals are also provided.
A method of producing a product that is produced upon expression of a metabolic pathway, comprising culturing a cell comprising an artificial chromosome under conditions whereby the pathway is expressed to produce the product, wherein:
A method for producing a gene product or products comprising:
A method for producing a gene product, comprising introducing a satellite artificial chromosome into a cell;
A method for producing a gene product or products, comprising culturing a cell containing a satellite artificial chromosome comprising DNA encoding the gene product or products under conditions whereby the gene product or products are expressed, wherein the satellite artificial chromosome is produced by a method, comprising:
Dependencies are inferred from the claim text.