Vaccine against mumps containing a Jeryl-Lynn virus strain
AssigneeSmithKline Beecham Biologicals s.a.
InventorsNigel Maurice Harford, Brigitte Desiree Alberte Colau, Jean Didelez
Life of this patent
Estimating the term…
Abstract
A new mumps vaccine is presented, comprising a homogeneous pure isolate derived from the Jeryl-Lynn strain of mumps virus. In a preferred embodiment of the invention the vaccine produces higher seroconversion and antibody titres than known commercial vaccines.
Claims20
An isolated, attenuated Jeryl-Lynn JL-1 mumps virus strain comprising the SH gene and the N terminus of the HN gene wherein said SH gene and said N terminus of the HN gene consist of the nucleic acid sequence as depicted in SEQ ID NO:1.
A vaccine comprising isolated Jeryl-Lynn JL-1 mumps virus strain comprising the SH gene and the N terminus of the HN gene wherein said SH gene and said N terminus of the HN gene consist of the nucleic acid sequence as set forth in SEQ ID NO:1.
A combined vaccine comprising:
- isolated Jeryl-Lynn JL-1 mumps virus strain comprising the SH gene and the N terminus of the HN gene wherein said SH gene and said N terminus of the HN gene consist of the nucleic acid sequence as set forth in SEQ ID NO:1;
- and at least one component selected from the group consisting of an attenuated measles virus, an attenuated rubella virus, an attenuated varicella zoster virus, a killed measles virus, a killed rubella virus, a killed varicella zoster virus and subunits of said viruses.
A method of producing an isolated Jeryl-Lynn mumps virus strain comprising the SH gene and the N terminus of the HN gene wherein said SH gene and said N terminus of the HN gene consist of the nucleic acid sequence as set forth in SEQ ID NO:1 and having less than 10% of any other Jeryl-Lynn strain, the method comprising:
- passaging a Jeryl-Lynn preparation comprising all of the characteristics of Accession No. V93110585 on a suitable cell line;
- and
- selecting a pure culture of an isolated Jeryl-Lynn mumps virus strain using the steps of either:
- a) limit dilution;
- or
- b) individual plaque isolation.
A method of inducing immunity in a mammal susceptible to mumps infection comprising:
- administering to the mammal an effective amount of a vaccine comprising isolated Jeryl-Lynn JL-1 mumps virus strain comprising the SH gene and the N terminus of the HN gene wherein said SH gene and the said N terminus of the HN gene consist of the nucleic acid sequence as set forth in SEQ ID NO:1.
A method of preventing infection comprising:
- administering to a mammal an effective amount of a vaccine comprising isolated Jeryl-Lynn JL-1 mumps virus strain comprising the SH gene and the N terminus of the HN gene wherein said SH gene and said N terminus of the HN gene consist of the nucleic acid sequence as set forth in SEQ ID NO:1.
A method of preventing infection comprising:
- administering to a mammal an effective amount of a combined vaccine comprising isolated Jeryl-Lynn JL-1 mumps virus strain comprising the SH gene and the N terminus of the HN gene wherein said SH gene and said N terminus of the HN gene consist of the nucleic acid sequence as set forth in SEQ ID NO:1 and at least one component selected from the group consisting of an attenuated measles virus, an attenuated rubella virus, an attenuated varicella zoster virus, a killed measles virus, a killed rubella virus, a killed varicella zoster virus and subunits of said viruses.
A vaccine comprising isolated Jeryl-Lynn JL-1 mumps virus strain comprising the SH gene and the N terminus of the HN gene wherein said SH gene and said N terminus of the HN gene consist of the nucleic acid sequence as set forth in SEQ ID NO:1 and having less than 10% of any other Jeryl-Lynn strain.
A combined vaccine comprising:
- isolated Jeryl-Lynn JL-1 mumps virus strain comprising the SH gene and the N terminus of the HN gene wherein said SH gene and said N terminus of the HN gene consist of the nucleic acid sequence as set forth in SEQ ID NO:1 and having less than 10% of any other Jeryl-Lynn strain;
- and
- at least one component selected from the group consisting of an attenuated measles virus, an attenuated rubella virus, an attenuated varicella zoster virus, a killed measles virus, a killed rubella virus, a killed varicella zoster virus and subunits of said viruses.
A method of inducing immunity in a mammal susceptible to mumps infection comprising:
- administering to the mammal an effective amount of a combined vaccine comprising isolated Jeryl-Lynn JL-1 mumps virus strain comprising the SH gene and the N terminus of the HN gene wherein said SH gene and said N terminus of the HN gene consist essentially of the nucleic acid sequence as set forth in SEQ ID NO:1 and at least one component selected from the group consisting of an attenuated measles virus, an attenuated rubella virus, an attenuated varicella zoster virus, a killed measles virus, a killed rubella virus, a killed varicella zoster virus and subunits of said viruses.
Dependencies are inferred from the claim text.