US 6,022,961 A · Fluorescent stain containing pyrylium salt and fluorescent staining method of biological sample

Abstract

A stain for a nucleic acid characterized by containing, as an effective component, a pyrylium salt compound having Y as a negative portion and a pyrylium ring or a pyrylium-similar ring, wherein the ring is comprised of X selected from O, S, Se and Te as a hetero atom and has substituents R.sup.1, R.sup.2 and R.sup.3, wherein each of R.sup.1 and R.sup.2 is independently a hydrogen atom, sulfonate group, amino group, substituted or unsubstituted aryl group, etc., R.sup.3 is -A or -L-A, L is -L.sup.1 -, -L.sup.2 -L.sup.3 - or -L.sup.4 -L.sup.5 -L.sup.6 -, and each of L.sup.1 to L.sup.6 is independently --(CH.dbd.CH)--, a divalent group derived from the substituted or unsubstituted aryl group, etc., A is a substituted or unsubstituted aryl group, etc., and Y.sup.- is an anion. A method for detecting a nucleic acid which comprises the steps of reacting a sample with the stain, and then detecting, by an optical means, the double-strand nucleic acid stained with the stain in the case that the double-strand nucleic acid is contained in the sample.

Claims18

Dependent claims

A probe for detecting a target single-stranded nucleic acid in a sample comprising:

a single-stranded nucleic acid having a base sequence being complementary to a base sequence of the target nucleic acid and a pyrylium compound represented by the following formula I covalently bound to the single-stranded nucleic acid ##STR58## wherein X is O, S, Se or Te, two of R.sup.1, R.sup.2 and R.sup.3 are each substituted or unsubstituted aryl groups;
the other is a hydrogen atom, halogen atom, sulfonate group, amino group, styryl group, nitro group, hydroxyl group, carboxyl group, cyano group, substituted or unsubstituted lower alkyl group, substituted or unsubstituted cycloalkyl group,
--A or --L--A,
L is --L.sup.1 --, --L.sup.2 --L.sup.3 -- or --L.sup.4 --L.sup.5 --L.sup.6 --, and each of L.sup.6 to L6 is independently .paren open-st.CH.dbd.CH.paren close-st., a divalent group derived from the substituted or unsubstituted aryl group, a substituted or unsubstituted lower alkylene group, or --CH.dbd.R.sup.4 --, wherein R.sup.4 is a ring structure having an oxo group,
A is a substituted or unsubstituted aryl group or --CH.dbd.R.sup.5 --, wherein R.sup.5 is a substituted or unsubstituted heterocyclic ring, a substituted or unsubstituted cycloalkyl group, or a substituted or unsubstituted aromatic ring,
and Y.sup.- is an anion.

3 more elements

Dependencies are inferred from the claim text.

Fluorescent stain containing pyrylium salt and fluorescent staining method of biological sample

Life of this patent

Fluorescent stain containing pyrylium salt and fluorescent staining method of biological sample

Life of this patent

Abstract

Claims18