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OwnerTakeshi Sano, Charles R. Cantor +4inventors
InventorsTakeshi Sano, Charles R. Cantor, Sandor Vajda, Gabriel O. Reznik, Cassandra L. Smith, Mark W. Pandori
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The present invention relates to streptavidin proteins and peptides having a altered physical properties such as an increased stability or increased or decreased affinity for binding biotin. The invention also relates to methods for the detection, identification, separation and isolation of targets using streptavidin proteins or peptides. Streptavidin with increased or reduced affinity allows for the use of the streptavidin-biotin coupling systems for detection and isolation systems wherein it is necessary to remove of one or the other of the binding partners. Such systems are useful for the purification of functional proteins and viable cells. The invention also relates to nucleic acids which encode these streptavidin proteins and peptides and to recombinant cells such as bacteria, yeast and mammalian cells which contain these nucleic acids.
A streptavidin protein comprising a subunit peptide consisting of SEQ ID NO 1 from positions 16 to 133, wherein said peptide has an amino acid substitution at position 127.
A streptavidin peptide consisting of SEQ ID NO 1 from positions 16 to 133, wherein said peptide has an amino acid substitution at position 127.
A streptavidin peptide consisting of SEQ ID NO 1 from positions 16 to 133 wherein said peptide has a deletion of the sequence from positions 113 to 120.
A biotin-binding streptavidin peptide comprising a portion of SEQ ID NO 1, said portion containing an aspartic acid at position 127.
A biotin-binding streptavidin peptide comprising a portion of SEQ ID NO 1, said portion containing a lysine at position 127.
A biotin-binding streptavidin peptide comprising a portion of SEQ ID NO 1, said portion containing a cysteine at position 127.
A streptavidin protein comprising a first streptavidin peptide, said first peptide comprising a portion of SEQ ID NO 1, said portion containing a lysine at position 127 and a second streptavidin peptide, said second peptide comprising a portion of SEQ ID NO 1, said portion containing an aspartic acid at position 127.
A streptavidin protein comprising a plurality of biotin-binding streptavidin subunit peptides, said subunit peptides comprising a portion of SEQ ID NO 1, said portion containing a cysteine at position 127.
Dependencies are inferred from the claim text.