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US 6,022,713 AGranted
Process for producing nucleoside 5'-triphosphates and application of the same
AssigneeYamasa Corporation
InventorsToshitada Noguchi, Toshikazu Shiba
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AssigneeYamasa Corporation
InventorsToshitada Noguchi, Toshikazu Shiba
Estimating the term…
The present invention relates to a process for producing nucleoside 5'-triphosphates (NTP) from nucleoside 5'-diphosphates (NDP) other than adenosine 5'-diphosphate (ADP), characterized by using a polyphosphate kinase as an enzyme and polyphosphate as the phosphate donor; and application of this process to various glycosylation reactions. This process makes it possible to conveniently and economically synthesize NTP from NDP enzymatically. Also, it becomes possible thereby to economically recycle and synthesize sugar nucleotides and synthesize, for example, oligosaccharides associating therewith without resort to expensive phosphoenol pyruvate, ATP, etc. in the reactions for reproducing or converting NDP into NTP in the systems for enzymatically synthesizing oligosaccharides by combining, for example, with the synthesis of sugar nucleotides.
A process for producing a nucleoside 5'-triphosphate (NTP) from a nucleoside 5'-diphosphate (NDP) other than adenosine 5'-diphosphate (ADP), comprising reacting a polyphosphate kinase with NDP and a polyphosphate, said polyphosphate serving as a phosphate donor.
A process for regenerating a NTP from a NDP, other than ADP, that have been produced from another enzymatic process, comprising reacting a polyphosphate kinase with NDP produced from another enzymatic process and a polyphosphate, said polyphosphate serving as a phosphate donor.
A process for glycosylating an acceptor sugar, comprising reacting a glycosyltransferase with a sugar nucleotide and an acceptor sugar to form the glycosylated acceptor sugar, said sugar nucleotide being produced from NTP which is produced by reacting a polyphosphate kinase with a nucleoside 5'-monophosphate (NMP) or NDP produced from the glycosylation reaction and a polyphosphate, said polyphosphate serving as a phosphate donor.
A process for recycling a NMP or a NDP, other than ADP, that have been produced from an enzymatic reaction, to a NTP, comprising reacting a polyphosphate kinase with NMP or NDP produced from an enzymatic reaction and a polyphosphate, said polyphosphate serving as a phosphate donor.
Dependencies are inferred from the claim text.