Article and method for detection of enterotoxigenic staphylococci
AssigneeMinnesota Mining and Manufacturing Company
InventorsPatrick A. Mach, Marlys E. Lund
Life of this patent
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Abstract
An article for detecting thermostable nuclease positive, potentially enterotoxigenic, staphylococci, containing unhydrolyzed nucleotides, toluidine blue O, and a binder, wherein the article is adapted for placement against a sample suspected of containing enterotoxigenic staphylococci. A method of detecting thermostable nuclease positive staphylococci in a sample utilizing the article, and a kit for the detection of thermostable nuclease positive staphylococci containing the article, are also described.
Claims20
A dry article for determining the presence or amount of thermostable nuclease positive staphylococci in a sample suspected of containing said thermostable nuclease positive staphylococci, said dry article comprising unhydrolyzed nucleotides, toluidine blue O, and a binder, said article further comprising at least two surfaces wherein at least one of said surfaces of said article is adapted for placement against said sample.
A method of determining the presence or amount of thermostable nuclease positive staphylococci in a cultured sample containing bacterial colonies, comprising the steps of:
- contacting a dry article for determining the presence or amount of said thermostable nuclease positive staphylococci with said sample, wherein said dry article comprises unhydrolyzed nucleotides, toluidine blue O, and a binder, further comprises at least two surfaces wherein at least one of said surfaces is adapted for placement against said sample, and wherein said sample comprises nutrient medium selective for growing staphylococci and has been heat-treated to inactivate non-thermostable nuclease activity;
- and
- determining the presence or amount of said thermostable nuclease positive staphylococci in said sample by detecting the presence or amount of a color change from blue to red or pink in said article.
A kit for determining the presence or amount of thermostable nuclease positive staphylococci in a sample, comprising reagents and nutrients selective for growing staphylococci from said sample, and a dry article for determining the presence or amount of thermostable nuclease positive staphylococci in said sample, wherein said dry article has at least two surfaces and comprises unhydrolyzed nucleotides, toluidine blue O, and a binder, and wherein at least one surface of said article is adapted for placement against said sample.
Dependencies are inferred from the claim text.