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US 6,022,477 AGranted
Method and apparatus for isolation purification of biomolecules
AssigneeNew Jersey Institute of Technology
InventorsRobert G. Luo, Kamalesh K. Sirkar
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Abstract
An apparatus and method for recovering bioproducts from a feed solution. In one embodiment, the apparatus includes a module housing, a membrane disposed in the housing for filtering the bioproducts from the feed solution, and an adsorbent bed disposed in the housing for retaining the bioproducts which permeate through the membrane, wherein the apparatus is adapted to allow fractionation and purification of the retained bioproducts from the bed by elution.
Claims15
A method of recovering bioproducts from a feed solution, the method comprising:
- providing a membrane module comprising a housing, at least one hollow fiber membrane disposed within the housing and having a feed side and a permeate side, a permeate outlet which extends outside of said housing, and a continuous packed bed of adsorbent particles in contact with the permeate side of said membrane, said particles being disposed within the housing and extending outside of said housing into said permeate outlet;
- passing the feed solution into the membrane module to contact the feed side of the membrane;
- separating bioproducts from the feed solution by permeation through to the permeate side of the membrane and allowing the permeated bioproducts to contact the adsorbent particles, wherein the bioproducts are retained by the adsorbent particles, whereby the bioproducts are isolated from the feed solution;
- and
- fractionating and purifying the retained bioproducts from the bed by chromatographic elution.
2 more elements
Dependencies are inferred from the claim text.