ribH

An isolated polynucleotide molecule comprising a nucleotide sequence that is complementary to a nucleotide sequence encoding a polypeptide having 6,7-dimethyl-8-ribityllumazine synthase activity, said nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:2.

An isolated polynucleotide molecule comprising a nucleotide sequence encoding a polypeptide having 6,7-dimethyl-8-ribityllumazine synthase activity, said nucleotide sequence having up to five nucleotide substitutions, deletions, or insertions for every 100 nucleotides of a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:2.

An isolated polynucleotide molecule comprising a nucleotide sequence encoding a polypeptide having 6,7dimethyl-8-ribityllumazine synthase activity, said nucleotide sequence having up to three nucleotide substitutions, deletions, or insertions for every 100 nucleotides of a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:2.

An isolated polynucleotide molecule comprising a nucleotide sequence encoding a polypeptide having 6,7-dimethyl-8-ribityllumazine synthase activity, said nucleotide sequence having up to five nucleotide substitutions, deletions, or insertions for every 100 nucleotides depicted in SEQ ID NO:

• 1.

An isolated polynucleotide molecule comprising a nucleotide sequence encoding a polypeptide having 6,7-dimethyl-8-ribityllumazine synthase activity, said nucleotide sequence having up to three nucleotide substitutions, deletions, or insertions for every 100 nucleotides depicted in SEQ ID NO:1.

An isolated polynucleotide molecule comprising a nucleotide sequence encoding a polypeptide having 6,7-dimethyl-8-ribityllumazine synthase activity, said nucleotide sequence encoding at least 50 amino acids depicted in SEQ ID NO:2.

An isolated polynucleotide molecule comprising a nucleotide sequence encoding a polypeptide having 6,7-dimethyl-8-ribityllumazine synthase activity, said nucleotide sequence encoding at least 30 amino acids depicted in SEQ ID NO:2.

An isolated polynucleotide molecule comprising a nucleotide sequence encoding the mature polypeptide expressed by the ribH gene contained in Streptococcus pneumoniae 0100993 contained in NCIMB Deposit No. 40794.

An isolated polynucleotide molecule comprising a nucleotide sequence which hybridizes to SEQ ID NO:

• 1, wherein the hybridization conditions include incubation at 42.degree. C. in a solution comprising 50% formamide, 5X SSC, 50 mM sodium phosphate (pH 7.6), 5.times.Denhartd's solution, 10% dextran sulfate, and 20 micrograms/ml denatured and sheared salmon sperm DNA, followed by washing in 0.1.times.SSC at about 65.degree. C., said nucleotide sequence encoding a polypeptide having 6,7-dimethyl-8-ribityllumazine synthase activity.

An isolated polynucleotide molecule comprising a nucleotide sequence which hybridizes to SEQ ID NO;

• 1 and to a DNA of a bacteria from same taxonomic order as Streptococcus pneumoniae, wherein the hybridization conditions include incubation at 42.degree. C. in a solution comprising 50% formamide, 5.times.SSC, 50 mM sodium phosphate (pH 7.6), 5.times.Denhartd's solution, 10% dextran sulfate, and 20 micrograms/ml denatured and sheared salmon sperm DNA, followed by washing in 0.1.times.SSC at about 65.degree. C.;
• and wherein the nucleotide sequence having up to five nucleotide substitutions, deletions, or insertions for every 100 nucleotides depicted in SEQ ID NO:
• 1.