Characterization of individual polymer molecules based on monomer-interface interactions
AssigneesThe United States of America as represented by the Secretary of Commerce, The Regents of the University of California
InventorsRichard Baldarelli, Daniel Branton, George Church, David W. Deamer, Mark Akeson, John Kasianowicz
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Abstract
A method for sequencing a nucleic acid polymer by (1) providing two separate, adjacent pools of a medium and an interface between the two pools, the interface having a channel so dimensioned as to allow sequential monomer-by-monomer passage from one pool to the other pool of only one nucleic acid polymer at a time; (2) placing the nucleic acid polymer to be sequenced in one of the two pools; and (3) taking measurements as each of the nucleotide monomers of the nucleic acid polymer passes through the channel so as to sequence the nucleic acid polymer.
Claims24
A method for sequencing a nucleic acid polymer, the method comprising:
- providing two separate, adjacent pools of a medium and an interface between the two pools, the interface having a channel so dimensioned as to allow sequential monomer-by-monomer passage from one pool to the other pool of only one nucleic acid polymer at a time;
- placing the nucleic acid polymer to be sequenced in one of the two pools;
- and
- taking measurements as each of the nucleotide monomers of the nucleic acid polymer passes through the channel so as to sequence the nucleic acid polymer.
Dependencies are inferred from the claim text.