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AssigneeMark A. Emalfarb
InventorsMark Aaron Emalfarb, Arie Ben-Bassat, Arkady Panteleimonovich Sinitsyn
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The subject invention relates to novel compositions of neutral and/or alkaline cellulase and methods for obtaining neutral and/or alkaline cellulase compositions from Chrysosporium cultures, in particular Chrysosporium lucknowense. This invention also provides mutants and methods of generating mutants of Chrysosporium capable of producing neutral and/or alkaline cellulase. This invention also relates to the genes encoding the enzymes comprising the neutral and/or alkaline cellulase composition. In addition, this invention provides methods of culturing Chrysosporium to produce neutral and/or alkaline cellulases. The neutral and/or alkaline cellulase compositions of the subject invention can be used in a variety of processes including stone washing of clothing, detergent processes, deinking and biobleaching of paper & pulp and treatment of waste streams.
A method of enzymatically treating cellulosic fibers or fabrics, comprising treating said fibers or fabrics with a neutral and/or alkaline cellulase whose amino acid sequence is encoded by a nucleic acid sequence from a fungus of the genus Chrysosporium, provided that, when said method is carried out at a pH below 7.0, said fungus is not Chrysosporium pruinosum.
A method of enzymatically treating cellulosic fibers or fabrics, comprising treating said fibers or fabrics with a neutral and/or alkaline cellulase isolated or obtained from a fungus of the genus Chrysosporium, provided that, when said method is carried out at a pH below 7.0, said fungus is not Chrysosporium pruinosum.
A method of stonewashing jeans, comprising treating said jeans with a neutral and/or alkaline cellulase whose amino acid sequence is encoded by a nucleic acid sequence from a fungus of the genus Chrysosporium, provided that, when said method is carried out at a pH below 7.0, said fungus is not Chrysosporium pruinosum.
A method of stonewashing jeans, comprising treating said jeans with a neutral and/or alkaline cellulase isolated or obtained from a fungus of the genus Chrysosporium, provided that, when said method is carried out at a pH below 7.0, said fungus is not Chrysosporium pruinosum.
A method of enhancing the softness or feel of cellulose- or cotton-containing fabrics, comprising treating said fabrics with a neutral and/or alkaline cellulase whose amino acid sequence is encoded by a nucleic acid sequence from a fungus of the genus Chrysosporium, provided that, when said method is carried out at a pH below 7.0, said fungus is not Chrysosporium pruinosum.
A method of enhancing the softness or feel of cellulose- or cotton-containing fabrics, comprising treating said fabrics with a neutral and/or alkaline cellulase isolated or obtained from a fungus of the genus Chrysosporium, provided that, when said method is carried out at a pH below 7.0, said fungus is not Chrysosporium pruinosum.
A method of enhancing the cleaning ability of a detergent composition, comprising adding to said detergent composition a neutral and/or alkaline cellulase whose amino acid sequence is encoded by a nucleic acid sequence from a fungus of the genus Chrysosporium, provided that, when said detergent composition has a a pH below 7.0, said fungus is not Chrysosporium pruinosum.
A method of enhancing the cleaning ability of a detergent composition, comprising adding to said detergent composition a neutral and/or alkaline cellulase isolated or obtained from a fungus of the genus Chrysosporium, provided that, when said detergent composition has a a pH below 7.0, said fungus is not Chrysosporium pruinosum.
A method of biopolishing, defibrillating, bleaching, dying, or desizing textiles, comprising treating said textiles with a neutral and/or alkaline cellulase whose amino acid sequence is encoded by a nucleic acid sequence from a fungus of the genus Chrysosporium, provided that, when said method is carried out at a pH below 7.0, said fungus is not Chrysosporium pruinosum.
A method of biopolishing, defibrillating, bleaching, dying, or desizing textiles, comprising treating said textiles with a neutral and/or alkaline cellulase isolated or obtained from a fungus of the genus Chrysosporium, provided that, when said method is carried out at a pH below 7.0, said fungus is not Chrysosporium pruinosum.
A method of deinking or biobleaching paper or pulp, comprising treating said paper or pulp with a neutral and/or alkaline cellulase whose amino acid sequence is encoded by a nucleic acid sequence from a fungus of the genus Chrysosporium, provided that, when said method is carried out at a pH below 7.0, said fungus is not Chrysosporium pruinosum.
A method of deinking or biobleaching paper or pulp, comprising treating said paper or pulp with a neutral and/or alkaline cellulase isolated or obtained from a fungus of the genus Chrysosporium, provided that, when said method is carried out at a pH below 7.0, said fungus is not Chrysosporium pruinosum.
A method of restoring color to textiles, comprising treating said textiles with a neutral and/or alkaline cellulase whose amino acid sequence is encoded by a nucleic acid sequence from a fungus of the genus Chrysosporium, provided that, when said method is carried out at a pH below 7.0, said fungus is not Chrysosporium pruinosum.
A method of restoring color to textiles, comprising treating said textiles with a neutral and/or alkaline cellulase isolated or obtained from a fungus of the genus Chrysosporium, provided that, when said method is carried out at a pH below 7.0, said fungus is not Chrysosporium pruinosum.
Dependencies are inferred from the claim text.