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AssigneeThe United States of America as represented by the Secretary of the Navy
InventorsStephen Alden Ralls, Lloyd Grant Simonson
Estimating the term…
An assay method and kit for detecting specific oral cariogenic bacteria, ., mutans streptococci, Lactobacillus sp. and Actinomyces sp., separately or in combination, comprising gathering a sample suspected of containing cariogenic bacteria; treating the sample with a stripping buffer to remove host antibodies from bacteria present in the sample; retaining the treated bacteria on a blocked solid phase substrate; reacting the retained bacteria with a primary antibody specific for the desired cariogenic bacteria; reacting the primary antibody with a conjugated label producing a detectable signal; and detecting the signal whereby the presence of the desired cariogenic bacteria is determined in the sample. The device for conducting these assays is a frame or support which holds a solid substrate capable of retaining the bacteria of interest while permitting drainage of other materials or fluids away from the retained bacteria.
A method for semiquantitatively detecting, in less than 30 minutes, a predesignated, target cariogenic bacteria in a sample selected from the group consisting of human dental plaque, saliva, and oral rinse which comprises the following steps:
A kit for semi-quantitatively detecting cariogenic bacteria in human dental plaque, saliva, and oral rinse samples comprising:
Dependencies are inferred from the claim text.