Method for the production of 1,3-propanediol by recombinant microorganisms
AssigneesE. I. Du Pont de Nemours and Company, Genencor International, Inc.
InventorsCharles E. Nakamura, Anthony A. Gatenby, Amy Kuang-Hua Hsu, Richard D. La Reau, Sharon L. Haynie, Maria Diaz-Torres, Donald E. Trimbur, Gregory M. Whited, Vasantha Nagarajan, Mark S. Payne, Stephen K. Picataggio, Ramesh V. Nair
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Abstract
Recombinant organisms are provided comprising genes encoding glycerol-3-phosphate dehydrogenase, glycerol-3-phosphatase, glycerol dehydratase and 1,3-propanediol oxidoreductase activites useful for the production of 1,3-propanediol from a variety of carbon substrates.
Claims13
A method for the production of 1,3-propanediol from a recombinant microorganism comprising:
- (i) transforming a suitable host microorganism with one or more transformation cassettes each of which comprises at least one of
- (a) a gene encoding a glycerol-3-phosphate dehydrogenase activity;
- (b) a gene encoding a glycerol-3-phosphatase activity;
- (c) genes encoding a dehydratase activity;
- and
- (d) a gene encoding 1,3-propanediol oxidoreductase activity,
- wherein all of the genes of (a)-(d) are introduced into the host microorganism;
- (ii) culturing the transformed host microorganism under suitable conditions in the presence of at least one carbon source selected from the group consisting of monosaccharides, oligosaccharides, polysaccharides, or a one-carbon substrate whereby 1,3-propanediol is produced;
- and
- (iii) recovering the 1,3-propanediol.
A method for the production of 1,3-propanediol from a recombinant microorganism comprising:
- (i) culturing, under suitable conditions in the presence of at least one carbon source selected from the group consisting of monosaccharides, oligosaccharides, polysaccharides, or a one-carbon substrate, a transformed host microorganism comprising
- (a) a gene encoding a glycerol-3-phosphate dehydrogenase activity;
- (b)a gene encoding a glycerol-3-phosphatase activity;
- (c)genes encoding a dehydratase activity;
- and
- (d) a gene encoding 1,3-propanediol oxidoreductase activity,
- wherein all of the genes (a)-(d) are exogenous to the host microorganism, whereby 1,3-propanediol is produced;
- and
- (ii) recovering the 1,3-propanediol.
A host cell transformed with a group of genes comprising:
- (1) a gene encoding a glycerol-3-phosphate dehydrogenase enzyme corresponding to the amino acid sequence given in SEQ ID NO:11;
- (2) a gene encoding a glycerol-3-phosphatase enzyme corresponding to the amino acid sequence given in SEQ ID NO:17;
- (3) a gene encoding the a subunit of the glycerol dehydratase enzyme corresponding to the amino acid sequence given in SEQ ID NO:34;
- (4) a gene encoding the .beta. subunit of the glycerol dehydratase enzyme corresponding to the amino acid sequence given in SEQ ID NO:35;
- (5) a gene encoding the .gamma. subunit of the glycerol dehydratase enzyme corresponding to the amino acid sequence given in SEQ ID NO:36;
- and
- (6) a gene encoding the 1,3-propanediol oxidoreductase enzyme corresponding to the amino acid sequence given in SEQ ID NO:37,
- whereby the transformed host cell produces 1,3-propanediol on at least one substrate selected from the group consisting of monosaccharides, oligosaccharides, and polysaccharides or from a one-carbon substrate.
Dependencies are inferred from the claim text.