Binding proteins for recognition of DNA
AssigneeMedical Research Council
InventorsYen Choo, Aaron Klug, Isidro Sanchez Garcia
Life of this patent
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Abstract
Disclosed are libraries of DNA sequences encoding zinc finger binding motifs for display on a particle, together with methods of designing zinc finger binding polypeptides for binding to a particular target sequence and, inter alia, use of designed zinc finger polypeptides for various in vitro or in vivo applications.
Claims26
A library of DNA sequences, each sequence encoding a zinc finger polypeptide for display on a viral particle, the zinc finger polypeptide comprising at least three zinc fingers, with one zinc finger having a partially randomized allocation of amino acids being positioned between two or more zinc fingers having a defined amino acid sequence, the partially randomized zinc finger having random allocation of amino acids at positions -1, +2, +3 and +6, position +1 being the first amino acid in the .alpha.-helix of the zing finger.
A kit for making a zinc finger polypeptide for binding to a DNA of interest, comprising:
- a library of DNA sequences, each sequence encoding a zinc finger polypedtide for display on a viral particle, the zinc finger polypeptide comprising at least three zinc fingers, with one zinc finger having a partially randomized allocation of amino acids being positioned between two or more zinc fingers having a defined amino acid sequence, the partially randomized zinc finger having random allocation of amino acids at positions -1, +2, +3 and +6. position +1 being the first amino acid in the .alpha.-helix of the zing finger;
- a vector molecule suitable for accepting one or more sequences from the library;
- and instructions for use.
Dependencies are inferred from the claim text.