Fungal promoters active in the presence of glucose
AssigneeRohm Enzyme Finland Oy
InventorsTiina Hannele Nakari, Maija-Leena Onnela, Marja Hannele Ilmen, Kaisu Milja Helena Nevalainen, Merja Elisa Penttila
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Abstract
A method is described for the identification and cloning of promoters that express under a defined environmental condition, such as growth in glucose medium. Using this method, five Trichodermal promoters capable of the high expression of operably linked coding sequences are identified, one of which is the promoter for T. reesei tef1. Also provided are altered cbh1 promoters, altered so that glucose no longer represses expression from such promoter. The invention further provides vectors and hosts that utilize such promoters, and unique fungal enzyme compositions from such hosts.
Claims38
An isolated promoter capable of expressing an operably-linked coding sequence in a fungal host grown on glucose wherein said promoter is selected from the group consisting of the Trichoderma tef1 promoter, the promoter of SEQ ID No. 2, the promoter of SEQ ID No. 3, the promoter of SEQ ID No. 4, the promoter of SEQ ID No. 5, the promoter of SEQ ID No. 6 and a derepressed cbh1 promoter, wherein said derepressed cbh1 promoter contains sequence alterations, relative to the native T. reesei cbh1 promoter (SEQ ID NO:
- 18), and wherein said derepressed cbh1 promoter has an increased ability to express said coding sequence when said fungal host is grown on said glucose relative to the ability of the native T. reesei cbh1 promoter to express said coding sequence when said same fungal host is grown on said glucose.
A promoter, wherein said promoter is selected from the cbh1 promoter of the group consisting of SEQ ID 19, SEQ ID 20, SEQ ID 21 and SEQ ID 22.
A glucose-derepressed promoter selected from the group consisting of the altered cbh1 promoter contained in DSM 11964, the altered cbh1 promoter contained in DSM 11965, and the altered cbh1 promoter contained in DSM 11966.
Dependencies are inferred from the claim text.