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US 6,008,036 AGranted
Method for purifying viruses by chromatography
AssigneePasteur Merieux Serums et Vaccins
InventorsBernard Fanget, Alain Francon
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Abstract
A method for purifying viruses from a cell line culture by chromatography, comprising an anion exchange chromatography step followed by a cation exchange chromatography step and optionally a metal-binding affinity chromatography step. The method is particularly suitable for producing viruses for use in vaccines.
Claims15
A method for purifying viruses obtained from a cell culture by ion exchange chromatography comprising
- introducing the viruses in cell culture medium onto a first chromatography exchanger,
- eluting the viruses of interest from the first chromatography exchanger,
- introducing the resulting eluate into a second chromatography exchanger, and
- eluting the viruses of interest from the second chromatography exchanger;
- wherein one of said first chromatography exchanger and said second chromatography exchanger is an anion exchanger and the other is a cation exchanger.
2 more elements
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