Binding proteins for recognition of DNA
AssigneeMedical Research Council
InventorsYen Choo, Aaron Klug, Isidro Sanchez Garcia
Life of this patent
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Abstract
Disclosed are libraries of DNA sequences encoding zinc finger binding motifs for display on a particle, together with methods of designing zinc finger binding polypeptides for binding to a particular target sequence and, inter alia, use of designed zinc finger polypeptides for various in vitro or in vivo applications.
Claims41
A library of DNA sequences, each sequence encoding a zinc finger polypeptide for display, the zinc finger polypeptide comprising at least one zinc finger having partially randomised allocation of amino acids, the partially randomised zinc finger having a random allocation of amino acids at positions -1, +2, +3 and +6 and at least one of positions +1, +5 or +8, position +1 being the first amino acid in the .alpha.-helix of the zinc finger.
A library of DNA sequences, each sequence encoding a zinc finger polypeptide for display, the zinc finger polypeptide comprising at least one zinc finger having partially randomised allocation of amino acids, the partially randomised zinc finger having a random allocation of amino acids at positions -1, +1, +2, +3 and +6, position +1 being the first amino acid in the .alpha.-helix of the zinc finger.
A library of DNA sequences, each sequence encoding a zinc finger polypeptide for display, the zinc finger polypeptide comprising at least one zinc finger having partially randomised allocation of amino acids, the partially randomised zinc finger having a random allocation of amino acids at positions -1, +2, +3, +5 and +6, position +1 being the first amino acid in the .alpha.-helix of the zinc finger.
A library of DNA sequences, each sequence encoding a zinc finger polypeptide for display, the zinc finger polypeptide comprising at least one zinc finger having partially randomised allocation of amino acids, the partially randomised zinc finger having a random allocation of amino acids at positions -1, +2, +3, +6 and +8, position +1 being the first amino acid in the .alpha.-helix of the zinc finger.
Dependencies are inferred from the claim text.