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US 6,004,773 AGranted
Method for producing L-lysine
AssigneeAjinomoto Co., Inc.
InventorsMasayuki Araki, Masakazu Sugimoto, Yasuhiko Yoshihara, Tsuyoshi Nakamatsu
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AssigneeAjinomoto Co., Inc.
InventorsMasayuki Araki, Masakazu Sugimoto, Yasuhiko Yoshihara, Tsuyoshi Nakamatsu
Estimating the term…
A coryneform bacterium harboring an aspartokinase in which feedback inhibition by L-lysine and L-threonine is substantially desensitized, and comprising an enhanced DNA sequence coding for a dihydrodipicolinate reductase, an enhanced DNA sequence coding for dihydropicolinate reductase, an enhance DNA sequence coding for dihydropicolinate synthase, an enhanced DNA sequence coding for diaminopimelate decarboxylase and an enhanced DNA sequence coding for aspartate aminotransferase; a method for producing L-lysine comprising the steps of cultivating the coryneform bacterium in an appropriate medium to allow L-lysine to be produced and accumulated in a culture of the bacterium, and collecting L-lysine from the culture; and a recombinant DNA usable for production of the coryneform bacterium.
A recombinant DNA autonomously replicable in cells of coryneform bacteria, comprising a DNA sequence coding for an aspartokinase in which feedback inhibition by L-lysine and L-threonine is desensitized, a DNA sequence coding for a dihydrodipicolinate reductase, a DNA sequence coding for dihydrodipicolinate synthase, a DNA sequence coding for diaminopimelate decarboxylase, and a DNA sequence coding for aspartate aminotransferase.
A coryneform bacterium harboring an aspartokinase in which feedback inhibition by L-lysine and L-threonine is desensitized, and comprising a DNA sequence coding for a dihydrodipicolinate reductase, a DNA sequence coding for dihydrodipicolinate synthase, a DNA sequence coding for diaminopimelate decarboxylase and a DNA sequence coding for aspartate aminotransferase, intracellular activities of the dihydrodipicolinate reductase, the dihydrodipicolinate synthase, the diaminopimelate decarboxylase and the aspartate aminotransferase being raised.
An isolated DNA coding for a protein comprising an amino acid sequence shown in SEQ ID NO:
A vector pVK7, which is autonomously replicable in cells of Escherichia coli and Brevibacterium lactofermentum, and comprising a multiple cloning site and lacZ'.
Dependencies are inferred from the claim text.