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US 6,002,072 AGranted
Coat protein gene for the FLA83 W strain of papaya ringspot virus
AssigneesSeminis Vegetable Seeds, Inc., Cornell Research Foundation, Inc.
InventorsRussell J. McMaster, Maury L. Boeshore, David M. Tricoli, John F. Reynolds, Kim J. Carney, Dennis Gonsalves
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Abstract
The present invention relates to a coat protein gene of papaya ringspot virus strain FLA83 W. This coat protein gene can be used to prepare plants which are resistant to papaya ringspot virus.
Claims15
An isolated and purified DNA molecule encoding the coat protein of the FLA83 W strain of papaya ringspot virus comprising the nucleotide sequence shown in SEQ ID NO:
- 1 or SEQ ID NO:4.
A method of preparing a papaya ringspot viral resistant plant comprising:
- (a) transforming plant cells with a chimeric expression cassette comprising a promoter functional in plant cells operably linked to a DNA molecule that encodes a coat protein, wherein the DNA molecule is from the papaya ringspot virus strain FLA83 W and wherein the DNA molecule comprises the nucleotide sequence shown in SEQ ID NO:1 or SEQ ID NO:4.
- (b) regenerating the plant cells to provide a differentiated plant;
- and
- (c) identifying a transformed plant that comprises the chimeric expression cassette and is resistant to infection by papaya ringspot virus.
1 more element
Dependencies are inferred from the claim text.