Isolated agarase enzyme from flavobacterium SP. strain NR19, cloned genes therefor, and expression thereof in transformed host cells
AssigneePromega Corporation
InventorsMark W. Knuth, Kimberly K. Knoche, Susanne Selman, James R. Hartnett
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Abstract
Novel purified agarase enzymes from Flavobacterium sp. strain NR19 and cloned genes encoding the agarase enzymes are disclosed. Transformed host cells which express the novel agarase enzymes in isolatable quantities are also described. Also disclosed are antibodies specifically reactive with the novel agarases.
Claims12
An isolated gene encoding an agarase enzyme having a molecular weight of about 42 kD by polyacrylamide gel electrophoresis, the gene being isolated from Flavobacterium sp. strain NR19.
An isolated gene encoding an agarase enzyme having a molecular weight of about 42 kD by polyacrylamide gel electrophoresis, wherein the agarase enzyme encoded by the gene contains an amino acid sequence as shown in SEQ. ID. NO:
- 17.
A recombinant DNA construct comprising a gene isolated from Flavobacterium sp. strain NR19 which encodes an agarase enzyme having a molecular weight of about 42 kD by polyacrylamide gel electrophoresis.
A recombinant plasmid vector for the expression of an agarase enzyme, the plasmid vector comprising a DNA insert isolated from Flavobacterium sp. strain NR19 and wherein the DNA insert encodes an agarase enzyme having a molecular weight of about 42 kD by polyacrylamide gel lectrophoresis.
A transformed host cell comprising a heterologous gene isolated from Flavobacterium sp. strain NR19 which encodes an agarase enzyme having a molecular weight of about 42 kD by polyacrylamide gel electrophoresis.
Dependencies are inferred from the claim text.