Rhamnogalacturonase, corresponding DNA sequence, rhamnogalacturonase containing enzyme preparation and use of the enzyme preparation

An isolated recombinant DNA sequence comprising a DNA sequence coding for a rhamnogalacturonase, wherein the rhamnogalacturonase is derived from a strain of Aspergillus aculeatus and:

• (a) has a pH-optimum of about 5.0;
• (b) retains at least 80% of the maximal activity throughout the pH range of 3-6.5;
• (c) has a temperature optimum of about 40.degree. C.;
• and
• (d) retains at least 80% of the maximal activity throughout the temperature range of 5-5.degree. C.

An isolated recombinant DNA sequence comprising a DNA sequence coding for an rhamnogalacturonase, wherein the rhamnogalacturonase has the following partial amino acid sequences:

• __________________________________________________________________________ Gly-Ala-Val-Gln-Gly-Phe-Gly-Tyr-Val-Tyr-His-Ala-Glu-Gly-Thr Tyr-Gly-Ala-Ar g (SEQ ID NO:
• 1) 1 5 10 15 - Ser-Xaa-Asn-Ile-Leu-Ser-Tyr-Gly-Ala-Val-Ala-Asp-Xaa-Ser-Thr- (SEQ ID NO:
• 2) 1 5 10 15 - Asp-Val-Gly-Pro-Ala-Ile-Thr-Ser-Ala-Xaa-Ala-Ala-Arg-Lys 20 25 - Ser-Arg-Asn-Ile (SEQ ID NO:
• 3) 1 - Ser-Ala-Tyr-Gly-Ser-Gly-Tyr-Xaa-Leu-Lys (SEQ ID NO:
• 4) 1 5 10 - Thr-Leu-Glu-Asp-Ile-Ala-Ile (SEQ ID NO:
• 5) 1 5 - Gly-Leu-Xaa-Ala-Xaa-Ile-Pro-Ile-Pro-Xaa-Ile-Pro-Pro-Xaa-Phe-Phe (SEQ ID NO:
• 6) 1 5 10 15 - Ser-Leu-Asp-Ile-Asp-Gly-Tyr (SEQ ID NO:
• 7) 1 5 - Ser-Val-His-Asp-Ile-Ile-Leu-Val-Asp-Ala-Pro-Ala-Phe (SEQ ID NO:
• 8) 1 5 10 - Ala-Ala-Asp-Leu-Ala- (SEQ ID NO:
• 9) 1 5 - Gly-Ser-Asn-Ile (SEQ ID NO:
• 10) 1 - Tyr-Pro-Gly-Leu-Thr-Pro-Tyr (SEQ ID NO:
• 11) 1 5 - and - Asn-Val-Tyr-Thr-Trp-Ser-Ser-Asn-Gln-Met-Tyr-Met-Ile-Lys (SEQ ID NO:
• 12). 1 5 10 __________________________________________________________________________

An isolated recombinant DNA sequence comprising a DNA sequence coding for a rhamnogalacturonase, wherein the rhamnogalacturonase is derived from a strain of Irpex lacteus and:

• (a) has a pH-optimum of about 5.5;
• (b) retains at least 80% of the maximal activity throughout the pH range of 3-12;
• (c) has a temperature optimum of about 40.degree. C.;
• and
• (d) retains at least 80% of the maximal activity throughout the temperature range of 10-17.degree. C.