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AssigneeTransgenomic, Inc.
InventorsDouglas T. Gjerde, Robert M. Haefele, David W. Togami
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Improved liquid chromatography systems having components made of titanium, stainless steel, or organic polymeric material are useful in the separation of polynucleotide fragments, particularly large fragments of double-stranded polynucleotides, by Matched Ion Polynucleotide Chromatography (MIPC). The titanium, stainless steel, or polymeric components are treated so that they do not release multivalent cations into aqueous solutions flowing through the chromatography system. Alternatively, or in addition to utilizing materials made of the components listed above, a multivalent cation capture resin placed upstream of the separation column can be employed to remove multivalent ions from the system. The multivalent cation capture resin can be contained in a guard disk, a guard column, or a guard cartridge. Novel methods for separating mixtures of polynucleotide fragments into fractions based on their molecular weight by Matched Ion Polynucleotide Chromatography and slalom chromatography utilize the liquid chromatographic systems described above.
A method for separating a mixture of polynucleotides, comprising flowing a solution of polynucleotides through a separation column containing Matched Ion Polynucleotide Chromatography separation particles, wherein the solution contains sufficient chelating agent to coat or passivate metal surfaces in the separation column and complex any multivalent cations in the solution.
A method for separating a mixture of polynucleotides, comprising flowing a solution of polynucleotides through a separation column containing silica Matched Ion Polynucleotide Chromatography separation particles, wherein the solution contains sufficient chelating agent to coat or passivate metal surfaces in the separation column and complex any multivalent cations in the solution.
Dependencies are inferred from the claim text.