US 5,994,126 A · Method for in vitro proliferation of dendritic cell precursors and their use to produce immunogens

Abstract

A method for producing proliferating cultures of dendritic cell precursors is provided. Also provided is a method for producing mature dendritic cells in culture from the proliferating dendritic cell precursors. The cultures of mature dendritic cells provide an effective means of producing novel T cell dependent antigens comprised of dendritic cell modified antigens or dendritic cells pulsed with antigen, including particulates, which antigen is processed and expressed on the antigen-activated dendritic cell. The novel antigens of the invention may be used as immunogens for vaccines or for the treatment of disease. These antigens may also be used to treat autoimmune diseases such as juvenile diabetes and multiple sclerosis.

Claims14

Dependent claims

A method of producing a population of mature dendritic cells from proliferating dendritic cell precursor cultures, comprising

a) providing a tissue source comprising dendritic cell precursors;
b) culturing the tissue source on a substrate and in culture medium to expand the number of dendritic cell precursors by allowing the dendritic cell precursors to proliferate;
wherein said culture medium comprises GM-CSF and at least one other factor which inhibits the proliferation or maturation of non-dendritic cell precursors thereby increasing the proportion of dendritic cell precursors in the culture;
and
c) continuing to culture the dendritic cell precursors for a period of time sufficient to allow them to mature into mature dendritic cells.

2 more elements

Dependencies are inferred from the claim text.

Facts

Application

08/261,537Filed 17 Jun 1994 · continuation in part

Priority

1 Apr 1992

Granted

30 Nov 1999

Term ends

Not estimatedThe source records don’t support an estimate.

Classification15 codes

C12N 5/0639
Cells from the blood or the immune system: dendritic cells, e.g. Langherhans cells in the epidermis
A61K 39/0008
Vertebrate antigens: antigens related to auto-immune diseases; Preparations to induce self-tolerance
A61K 40/19
Characterised by the cell type used: dendritic cells
A61K 40/22
Characterised by the effect or the function of the cells: immunosuppressive or immunotolerising

Method for in vitro proliferation of dendritic cell precursors and their use to produce immunogens

Life of this patent

Method for in vitro proliferation of dendritic cell precursors and their use to produce immunogens

Life of this patent

Abstract

Claims14