US 5,993,844 A · Chemical treatment, without detergents or enzymes, of tissue to form an acellular, collagenous matrix

Abstract

The invention is directed to collagenous tissues which have been treated to remove non-collagenous components such as cells, cellular debris, and other extracellular matrix components, such as proteoglycans and glycosaminoglycans, normally found in native tissues. Treatment of the tissue with alkali, chelating agents, acids and salts removes non-collagenous components from the collagenous tissue matrix while controlling the amount of swelling and dissolution so that the resultant collagen matrix retains its structural organization, integrity and bioremodelable properties. The process circumvents the need to use detergents and enzymes which detrimentally affect the cell compatibility, strength and bioremodelability of the collagen matrix. The collagenous tissue matrix is used for implantation, repair, or use in a mammalian host.

Claims23

Dependent claims

A detergent-free and enzyme-free method for the removal of non-collagenous components from native mammalian tissue to yield an essentially collagenous tissue matrix that maintains its structural integrity, comprising:

(a) contacting the tissue with an alkaline solution containing a chelating agent selected from the group consisting of ethylenediaminetetraacetic acid and ethylenebis(oxyethylenitrilo)tetraacetic acid wherein the concentration of the chelating agent in the alkaline solution is between about 1 to about 200 mM and wherein the pH of the solution is between about 8 to about 12;
(b) contacting the tissue with an acidic solution containing a salt wherein the concentration of the salt in the acidic solution is between about 0.1 to about 2 M and wherein the pH of the solution is between 0 and about 1;
(c) contacting the tissue with a salt solution with a pH between about 5 and about 9;
and,
(d) contacting the tissue with a rinse agent, wherein the tissue maintains its structural integrity.

2 more elements

Dependencies are inferred from the claim text.

Chemical treatment, without detergents or enzymes, of tissue to form an acellular, collagenous matrix

Life of this patent

Chemical treatment, without detergents or enzymes, of tissue to form an acellular, collagenous matrix

Life of this patent

Abstract

Claims23